Investigation of size-dependent cell adhesion on nanostructured interfaces.
Investigation of size-dependent cell adhesion on nanostructured interfaces.
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DOI：
10.1186/s12951-014-0054-4
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发表时间：
2014-12-05
影响因子：
10.2
通讯作者：
Chen P
中科院分区：
文献类型：
--
作者：
Kuo CW;Chueh DY;Chen P
细胞通过膜结合受体探索材料表面，如整合素，并利用它们与吸附在底物表面的细胞外基质分子相互作用，导致局部粘连的形成。随着纳米技术的最新进展，生物传感器和生物电子学正在以越来越小的特征尺寸被制造出来。这些器件的性能取决于电池如何与器件表面的纳米结构相互作用。然而，细胞在纳米结构上的行为还没有完全被理解。在这里，我们提出了一个系统的研究细胞-纳米结构相互作用使用不同直径的聚合物纳米管。我们首先检查了生长在直径从200纳米到700纳米的纳米柱上的细胞的活力。观察到，当细胞在纳米管上培养时，随着纳米管尺寸的减小，细胞凋亡率略有增加。然后，我们使用共聚焦显微镜计算了灶性粘连的平均大小和灶性粘连的细胞铺展面积。研究发现，随着纳米柱尺寸的减小，在纳米柱上形成的焦点粘连的尺寸也会减小，这类似于新生的焦点复合体的形成。然而，当纳米微管的尺寸减小到200 nm时，焦点粘连的尺寸增加。进一步的研究表明，细胞与直径为200 nm的纳米管相互作用非常强烈，并施加足够的力使纳米管弯曲在一起，导致更大的焦点粘连形成。我们开发了一种简单的方法，使用尺寸可调的聚合物纳米柱在物理上定义良好的基质上系统地研究细胞-基质之间的相互作用。从这项研究中，我们得出结论，细胞可以在凋亡率略有增加的情况下在纳米结构上存活，并且细胞与更小的纳米结构之间的相互作用非常强烈。与之前在平面衬底上观察到的细胞与较软衬底之间的弱相互作用不同，我们在直径较小的较软纳米管上观察到了较强的细胞-衬底相互作用。我们的结果表明，除了衬底的刚性之外，纳米结构的尺寸也是可以用来调节细胞行为的额外的重要物理参数。
Cells explore the surfaces of materials through membrane-bound receptors, such as the integrins, and use them to interact with extracellular matrix molecules adsorbed on the substrate surfaces, resulting in the formation of focal adhesions. With recent advances in nanotechnology, biosensors and bioelectronics are being fabricated with ever decreasing feature sizes. The performances of these devices depend on how cells interact with nanostructures on the device surfaces. However, the behavior of cells on nanostructures is not yet fully understood. Here we present a systematic study of cell-nanostructure interaction using polymeric nanopillars with various diameters. We first checked the viability of cells grown on nanopillars with diameters ranging from 200 nm to 700 nm. It was observed that when cells were cultured on the nanopillars, the apoptosis rate slightly increased as the size of the nanopillar decreased. We then calculated the average size of the focal adhesions and the cell-spreading area for focal adhesions using confocal microscopy. The size of focal adhesions formed on the nanopillars was found to decrease as the size of the nanopillars decreased, resembling the formations of nascent focal complexes. However, when the size of nanopillars decreased to 200 nm, the size of the focal adhesions increased. Further study revealed that cells interacted very strongly with the nanopillars with a diameter of 200 nm and exerted sufficient forces to bend the nanopillars together, resulting in the formation of larger focal adhesions. We have developed a simple approach to systematically study cell-substrate interactions on physically well-defined substrates using size-tunable polymeric nanopillars. From this study, we conclude that cells can survive on nanostructures with a slight increase in apoptosis rate and that cells interact very strongly with smaller nanostructures. In contrast to previous observations on flat substrates that cells interacted weakly with softer substrates, we observed strong cell-substrate interactions on the softer nanopillars with smaller diameters. Our results indicate that in addition to substrate rigidity, nanostructure dimensions are additional important physical parameters that can be used to regulate behaviour of cells.
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影响因子：
13.3
作者：
Hsiao, Yu-Sheng;Luo, Shyh-Chyang;Hou, Shuang;Zhu, Bo;Sekine, Jun;Kuo, Chiung-Wen;Chueh, Di-Yen;Yu, Hsiao-hua;Tseng, Hsian-Rong;Chen, Peilin
通讯作者：
Chen, Peilin
DOI：
10.1038/nrm2957
发表时间：
2010-09
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
通讯作者：
--
影响因子：
29.4
作者：
Kuo, CW;Shiu, JY;Chen, P
通讯作者：
Chen, P
影响因子：
56.9
作者：
Lee, KB;Park, SJ;Mrksich, M
通讯作者：
Mrksich, M
影响因子：
4.9
作者：
Liliensiek, S. J.;Campbell, S.;Murphy, C. J.
通讯作者：
Murphy, C. J.