Vibrational spectroscopy and microscopic imaging: novel approaches for comparing barrier physical properties in native and human skin equivalents
Vibrational spectroscopy and microscopic imaging: novel approaches for comparing barrier physical properties in native and human skin equivalents
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振动光谱和显微成像：比较天然皮肤和人类皮肤等效物的屏障物理特性的新方法
DOI：
10.1117/1.jbo.18.6.061207
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发表时间：
2012
影响因子：
3.5
通讯作者：
R. Mendelsohn
中科院分区：
文献类型：
--
作者：
Guo Yu;Guojin Zhang;C. Flach;R. Mendelsohn
抽象的。振动光谱和成像已用于比较人皮肤、猪皮和两种人皮肤等同物（具有增强屏障的Epidemax 200X和具有正常屏障的Epidemax 200）的屏障特性。进行了三种结构表征。首先，在分离的人角质层（SC）、分离的猪SC和Epidermal 200X表面层中比较链包装和构象顺序。分离的人SC的红外（IR）光谱揭示了大比例的正交堆积的脂质链在生理温度下沿着与热致相变的状态与六边形堆积链。相比之下，在生理温度下，在猪SC和Epidermal 200X的脂质相，虽然占主导地位的构象有序的链，缺乏显着水平的正交亚细胞包装。第二，胆固醇条带的共焦拉曼成像显示在人类皮肤等同物（HSE）内广泛形成富含胆固醇的口袋。最后，IR成像跟踪脂质屏障尺寸以及有序脂质在人SC和Epidermal 200X中的空间分布。这些方法提供了一组有用的实验，用于探索离体人皮肤和HSE之间的结构差异，这反过来又可以为这些制剂中观察到的功能差异提供依据。
Abstract. Vibrational spectroscopy and imaging have been used to compare barrier properties in human skin, porcine skin, and two human skin equivalents, Epiderm 200X with an enhanced barrier and Epiderm 200 with a normal barrier. Three structural characterizations were performed. First, chain packing and conformational order were compared in isolated human stratum corneum (SC), isolated porcine SC, and in the Epiderm 200X surface layers. The infrared (IR) spectrum of isolated human SC revealed a large proportion of orthorhombically packed lipid chains at physiological temperatures along with a thermotropic phase transition to a state with hexagonally packed chains. In contrast, the lipid phase at physiological temperatures in both porcine SC and in Epiderm 200X, although dominated by conformationally ordered chains, lacked significant levels of orthorhombic subcell packing. Second, confocal Raman imaging of cholesterol bands showed extensive formation of cholesterol-enriched pockets within the human skin equivalents (HSEs). Finally, IR imaging tracked lipid barrier dimensions as well as the spatial disposition of ordered lipids in human SC and Epiderm 200X. These approaches provide a useful set of experiments for exploring structural differences between excised human skin and HSEs, which in turn may provide a rationale for the functional differences observed among these preparations.
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影响因子：
6.5
作者：
BOMMANNAN, D;POTTS, RO;GUY, RH
通讯作者：
GUY, RH
DOI：
10.1038/sj.jid.5700690
发表时间：
2007-05
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Guojin Zhang;D. Moore;K. Sloan;C. Flach;R. Mendelsohn
通讯作者：
Guojin Zhang;D. Moore;K. Sloan;C. Flach;R. Mendelsohn
影响因子：
3.5
作者：
Yeh, AT;Kao, BS;Tromberg, BJ
通讯作者：
Tromberg, BJ
胆固醇对合成神经酰胺 2 和十八烷酸二元混合物中混溶性和相行为的影响。
DOI：
10.1016/s0005-2736(01)00339-x
发表时间：
2001
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Chen,H;Mendelsohn,R;Rerek,ME;Moore,DJ
通讯作者：
Moore,DJ
膜筏形成动力学：角质层脂质模型中的脂肪酸结构域。
DOI：
10.1021/jp054875h
发表时间：
2006
期刊：
The journal of physical chemistry. B
影响因子：
--
作者：
Moore,DavidJ;Snyder,RobertG;Rerek,MarkE;Mendelsohn,Richard
通讯作者：
Mendelsohn,Richard