Lipopolysaccharide derived from the rumen down-regulates stearoyl-CoA desaturase 1 expression and alters fatty acid composition in the liver of dairy cows fed a high-concentrate diet.
Lipopolysaccharide derived from the rumen down-regulates stearoyl-CoA desaturase 1 expression and alters fatty acid composition in the liver of dairy cows fed a high-concentrate diet.
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源自瘤胃的脂多糖下调硬脂酰辅酶A去饱和酶 1 的表达并改变高浓缩饲料奶牛肝脏中的脂肪酸组成
DOI：
10.1186/s12917-015-0360-6
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发表时间：
2015-03-07
影响因子：
2.6
通讯作者：
Shen X
中科院分区：
文献类型：
--
作者：
Xu T;Tao H;Chang G;Zhang K;Xu L;Shen X
背景奶牛常饲喂高精料日粮以满足泌乳需求，但长期饲喂精料会引起亚急性瘤胃酸中毒（SARA）并导致乳脂下降。硬脂酰辅酶A去饱和酶1（Stearoyl-CoA desaturase 1，SCD 1）参与哺乳反刍动物肝脏脂肪酸的生物合成。在这里，我们进行了这项研究，以调查来自瘤胃的脂多糖对SCD 1的表达和脂肪酸组成的奶牛饲喂高浓缩饲料的肝脏的影响。选用8头经产泌乳中期荷斯坦奶牛（455 ± 28 kg），随机分为2组，分别饲喂低精料（LC）和高精料（HC）日粮18周。在饲喂HC饲料的动物中，瘤胃和肝静脉中的长链脂肪酸谱发生了显著改变。血浆中甘油三酯（TG）、非酯化脂肪酸（NEFA）和总胆固醇（TCH）含量显著降低，而血糖和胰岛素水平升高。HC组肝组织中SCD 1的表达明显下调。关于转录调节因子，固醇调节元件结合转录因子的表达（SREBF 1c，SREBF 2）和SREBP裂解激活蛋白（SCAP）表达下调，而过氧化物酶体增殖物激活受体α（peroxisome proliferator-activated receptor α，PPARα）表达上调。CoA去饱和酶1的表达和改变高浓度日粮奶牛肝脏中的脂肪酸组成
BackgroundDairy cows are often fed a high-concentrate diet to meet lactating demands, yet long-term concentrate feeding induces subacute ruminal acidosis (SARA) and leads to a decrease in milk fat. Stearoyl-CoA desaturase1 (SCD1) participates in fatty acid biosynthesis in the liver of lactating ruminants. Here, we conducted this study to investigate the impact of lipopolysaccharide derived from the rumen on SCD1 expression and on fatty acid composition in the liver of dairy cows fed a high-concentrate diet. Eight multiparous mid-lactating Holstein cows (455 ± 28 kg) were randomly assigned into two groups in the experiment and were fed a low-concentrate diet (LC) or high-concentrate diet (HC) for 18 weeks.ResultsThe results showed that the total volatile fatty acids and lactic acid accumulated in the rumen, leading to a decreased rumen pH and elevated lipopolysaccharides (LPSs) in the HC group. The long chain fatty acid profile in the rumen and hepatic vein was remarkably altered in the animals fed the HC diet. The triglyceride (TG), non-esterified fatty acid (NEFA) and total cholesterol (TCH) content in the plasma was significantly decreased, whereas plasma glucose and insulin levels were increased. The expression of SCD1 in the liver was significantly down-regulated in the HC group. In regards to transcriptional regulators, the expression of sterol regulatory element binding transcription factors (SREBF1c, SREBF2) and SREBP cleavage activating protein (SCAP) was down-regulated, while peroxisome proliferator-activated receptor α (PPARα) was up-regulated.ConclusionsThese data indicate that lipopolysaccharide derived from the rumen down-regulates stearoyl-CoA desaturase 1 expression and alters fatty acid composition in the liver of dairy cows fed a high-concentrate diet.
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影响因子：
3.5
作者：
Fairfield, A. M.;Plaizier, J. C.;McBride, B. W.
通讯作者：
McBride, B. W.
影响因子：
7.7
作者：
Biddinger, SB;Almind, K;Kahn, CR
通讯作者：
Kahn, CR
影响因子：
3.5
作者：
Douglas, G. N.;Rehage, J.;Drackley, J. K.
通讯作者：
Drackley, J. K.
DOI：
10.4137/grsb.s12005
发表时间：
2013
期刊：
Gene regulation and systems biology
影响因子：
--
作者：
Akbar H;Schmitt E;Ballou MA;Corrêa MN;Depeters EJ;Loor JJ
通讯作者：
Loor JJ
影响因子：
3.3
作者：
Blanch, M.;Calsamiglia, S.;Wallace, R. J.
通讯作者：
Wallace, R. J.