Impact of contrasting poultry exposures on human, poultry, and wastewater antibiotic resistomes in Bangladesh.
Impact of contrasting poultry exposures on human, poultry, and wastewater antibiotic resistomes in Bangladesh.
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DOI：
10.1128/spectrum.01763-23
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发表时间：
2023-12-12
影响因子：
3.7
通讯作者：
Islam, Mohammad Aminul
中科院分区：
文献类型：
--
作者：
Williams, Alexander D.;Rousham, Emily;Neal, Andrew L.;Amin, Mohammed Badrul;Hobman, Jon L.;Stekel, Dov;Islam, Mohammad Aminul
人类、动物和环境之间的相互作用被认为是解决抗生素耐药性（AMR）的关键焦点。然而，One Health关于低收入和中等收入国家抗生素耐药性的数据目前很少。使用宏基因组学，我们调查了人类粪便抗生素耐药性是否以及如何受到孟加拉国城市湿货市场（n = 13）和农村家庭（n = 7）对比环境中密集和非密集饲养家禽的影响。我们还考虑了家禽（n = 10）和废水（n = 10）在这些环境中的耐药。我们发现，职业性家禽暴露并没有显着改变人类粪便抵抗组。与此相反，大环内酯-林可酰胺-链阳性菌素和链丝菌素抗生素耐药基因（ARGs）丰富的家禽从城市湿货市场相对于农村家庭的鸡。废水中的ARG含量最高，但这只能部分解释家禽盲肠和人类粪便来源。废水中还含有具有临床意义的碳青霉烯类ARG。因此，这项研究提供了关键的洞察孟加拉国的ARG的分布。通过使用DNA测序，我们的研究表明，在从高暴露于常规喂养抗生素的家禽的个体和没有这种暴露的个体采集的粪便样本中发现的抗生素耐药基因没有显著差异。这一发现意义重大，因为它表明在这些情况下家禽和人类之间抗生素耐药基因的传播有限。然而，我们的研究还表明，商业饲养的家禽更有可能拥有对中型农场常用抗生素的耐药基因。此外，我们的研究强调了废水作为各种抗生素耐药基因来源的潜力，其中一些与临床相关。
Interactions between humans, animals, and the environment are considered critical foci for addressing antimicrobial resistance (AMR). However, One Health data on AMR in low- and middle-income countries are presently scarce. Using metagenomics, we investigated whether and how the fecal antibiotic resistomes of humans are influenced by exposure to intensive and non-intensively reared poultry within contrasting settings of urban wet markets (n = 13) and rural households (n = 7) in Bangladesh. We also considered poultry (n = 10) and wastewater (n = 10) resistomes in these settings. We found that occupational poultry exposures did not significantly alter the human fecal resistome. In contrast, macrolide-lincosamide-streptogramin and streptothricin antibiotic resistance genes (ARGs) were enriched in poultry from urban wet markets relative to rural household chickens. Wastewater had the highest ARG richness, though this was only partially explained by poultry cecal and human fecal sources. Wastewater also contained clinically significant carbapenem ARGs. This study therefore provides critical insight into the distribution of ARGs in Bangladesh. Through the use of DNA sequencing, our study shows that there is no significant difference in the antibiotic resistance genes found in stool samples taken from individuals with high exposure to poultry routinely fed antibiotics and those without such exposure. This finding is significant as it suggests limited transmission of antibiotic resistance genes between poultry and humans in these circumstances. However, our research also demonstrates that commercially reared poultry are more likely to possess resistance genes to antibiotics commonly administered on medium-sized farms. Additionally, our study highlights the under-explored potential of wastewater as a source of various antibiotic resistance genes, some of which are clinically relevant.
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影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
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影响因子：
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