Effect of p38 inhibitor on the proliferation of chicken muscle stem cells and differentiation into muscle and fat.
Effect of p38 inhibitor on the proliferation of chicken muscle stem cells and differentiation into muscle and fat.
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DOI：
10.5713/ab.22.0171
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发表时间：
2023-03
影响因子：
2.2
通讯作者：
Jo, Cheorun
中科院分区：
文献类型：
--
作者：
Ryu, Minkyung;Kim, Minsu;Jung, Hyun Young;Kim, Cho Hyun;Jo, Cheorun
在大多数物种中，抑制 p38 丝裂原激活蛋白激酶 (MAPK) 信号通路可延迟分化并增加肌肉干细胞的增殖。在这里，我们的目的是研究p38抑制剂（p38i）处理对鸡肌肉干细胞增殖和分化的影响。鸡肌肉干细胞从胚胎第18天的Hy-line Brown鸡胚胎的肌肉组织中采集，然后通过预平板法分离。细胞在补充有二甲基亚砜或 1、10、20 μM p38i 的生长培养基中培养 4 天，然后传代培养最多 4 代。用分化培养基诱导分化3天。每个处理重复3次。 p38i处理的培养物中配对盒7基因和肌原因子5基因的增殖和mRNA表达，以及肌原分化标记基因肌细胞生成素的mRNA表达显着高于对照组（p<0.05），但免疫荧光染色和肌球蛋白重链（MHC）的mRNA表达在两组之间没有显着差异。对分化细胞培养物中积累的脂滴进行油红 O 染色显示，p38i 处理的培养物中的脂质密度高于对照培养物；然而，脂肪形成标记基因过氧化物酶体增殖物激活受体γ的表达在两组之间没有显着差异。鸡肌肉干细胞中的 p38 抑制可改善细胞增殖，但对生肌分化和脂质积累的影响需要进一步分析。需要对鸡 p38-MAPK 通路进行进一步研究，以了解肌肉和脂肪发育机制。
Inhibiting the p38 mitogen-activated protein kinase (MAPK) signaling pathway delays differentiation and increases proliferation of muscle stem cells in most species. Here, we aimed to investigate the effect of p38 inhibitor (p38i) treatment on the proliferation and differentiation of chicken muscle stem cells. Chicken muscle stem cells were collected from the muscle tissues of Hy-line Brown chicken embryos at embryonic day 18, then isolated by the preplating method. Cells were cultured for 4 days in growth medium supplemented with dimethyl sulfoxide or 1, 10, 20 μM of p38i, then subcultured for up to 4 passages. Differentiation was induced for 3 days with differentiation medium. Each treatment was replicated 3 times. The proliferation and mRNA expression of paired box 7 gene and myogenic factor 5 gene, as well as the mRNA expression of myogenic differentiation marker gene myogenin were significantly higher in p38i-treated cultures than in control (p<0.05), but immunofluorescence staining and mRNA expression of myosin heavy chain (MHC) were not significantly different between the two groups. Oil red O staining of accumulated lipid droplets in differentiated cell cultures revealed a higher lipid density in p38i-treated cultures than in control; however, the expression of the adipogenic marker gene peroxisome proliferator activated receptor gamma was not significantly different between the two groups. p38 inhibition in chicken muscle stem cells improves cell proliferation, but the effects on myogenic differentiation and lipid accumulation require additional analysis. Further studies are needed on the chicken p38-MAPK pathway to understand the muscle and fat development mechanism.
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影响因子：
7
作者：
Ding S;Wang F;Liu Y;Li S;Zhou G;Hu P
通讯作者：
Hu P
影响因子：
--
作者：
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通讯作者：
Guan, Weijun
影响因子：
11.1
作者：
Wang C;Liu W;Nie Y;Qaher M;Horton HE;Yue F;Asakura A;Kuang S
通讯作者：
Kuang S
影响因子：
3.9
作者：
Beier, Justus P.;Bitto, Franz F.;Kneser, Ulrich
通讯作者：
Kneser, Ulrich
DOI：
10.1152/ajpregu.00087.2010
发表时间：
2011-07-01
影响因子：
2.8
作者：
Joubert, Romain;Metayer-Coustard, Sonia;Collin, Anne
通讯作者：
Collin, Anne