DSPP effects on in vivo bone mineralization.
DSPP effects on in vivo bone mineralization.
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DSPP 对体内骨矿化的影响。
DOI：
10.1016/j.bone.2008.08.110
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发表时间：
2008-12
期刊：
影响因子：
4.1
通讯作者：
Boskey, Adele L.
中科院分区：
文献类型：
--
作者：
Verdelis, Kostas;Ling, Yunfeng;Sreenath, Taduru;Haruyama, Naoto;MacDougall, Mary;van der Meulen, Marjolein C. H.;Lukashova, Lyudmila;Spevak, Lyudmila;Kulkarni, Ashok B.;Boskey, Adele L.
牙本质涎磷蛋白参与了Dspp基因敲除小鼠（Dspp-/-）牙本质形成缺陷的矿化过程。Dspp在骨中以低水平表达，并且通过定量显微计算机断层扫描（microCT）和傅立叶变换红外光谱成像（FTIRI）评估的Dspp-/-股骨在发育和成熟小鼠中均表现出与野生型股骨的一些矿物质和基质性质差异。与野生型相比，Dspp-/-小鼠最初（5周）和7个月时的骨小梁体积分数显著较高，骨小梁分离较低，而在9个月时，骨体积分数和骨小梁数量较低。Dspp-/-的皮质骨矿物质密度、面积和惯性矩在9个月时降低。通过FTIRI，Dspp-/-动物最初（5个月）含有更多的化学计量骨磷灰石，具有更高的结晶度（晶体尺寸/完整性）和更低的碳酸盐取代。这种差异随着年龄的增长而逐渐逆转（到9个月龄时，Dspp-/-皮质骨中的结晶度显著降低，酸性磷酸盐含量增加）。在个体皮质骨和松质骨中，3D micro-CT测定的矿物质密度和2D FTIRI测定的矿物质与基质比率相关（r2=0.6，p<0.04）。从矩阵分析，皮质骨和松质骨的胶原成熟度在5周时Dspp-/-组大于对照组;到9个月时，交联模式不存在这种差异。在不同年龄段观察到的矿物质和基质性质的变化，部分归因于Dspp基因产物调节初始矿化和重塑的能力，这意味着Dspp对骨转换的影响。
Dentin sialophosphoprotein has been implicated in the mineralization process based on the defective dentin formation in Dspp null mice (Dspp-/-). Dspp is expressed at low levels in bone and Dspp-/- femurs assessed by quantitative micro-computed tomography (microCT) and Fourier transform infrared spectroscopic imaging (FTIRI) exhibit some mineral and matrix property differences from wildtype femurs in both developing and mature mice. Compared to wildtype, Dspp-/- mice initially (5 weeks) and at 7 months had significantly higher trabecular bone volume fractions and lower trabecular separation, while at 9 months, bone volume fraction and trabecular number were lower. Cortical bone mineral density, area, and moments of inertia in Dspp-/- were reduced at 9 months. By FTIRI, Dspp-/- animals initially (5 months) contained more stoichiometric bone apatite with higher crystallinity (crystal size/perfection) and lower carbonate substitution. This difference progressively reversed with age (significantly decreased crystallinity and increased acid phosphate content in Dspp-/- cortical bone by 9 months of age). Mineral density as determined in 3D micro-CT and mineral-to-matrix ratios as determined by 2D FTIRI in individual cortical and trabecular bones were correlated (r2=0.6, p<0.04). From the matrix analysis, the collagen maturity of both cortical and trabecular bone was greater in Dspp-/- than controls at 5 weeks; by 9 months this difference in cross-linking pattern did not exist. Variations in mineral and matrix properties observed at different ages are attributable, in part, to the ability of the Dspp gene products to regulate both initial mineralization and remodeling, implying an effect of Dspp on bone turnover.
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影响因子：
2.9
作者：
Butler, WT;Brunn, JC;McKee, MD
通讯作者：
McKee, MD
影响因子：
4.2
作者：
Gericke, A;Qin, C;Boskey, AL
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2.9
作者：
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通讯作者：
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4.2
作者：
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作者：
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通讯作者：
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