Association of Randall plaque with collagen fibers and membrane vesicles.
Association of Randall plaque with collagen fibers and membrane vesicles.
复制标题
DOI：
10.1016/j.juro.2011.10.125
复制
发表时间：
2012-03
期刊：
影响因子：
--
通讯作者：
Monga M
中科院分区：
文献类型：
--
作者：
Khan SR;Rodriguez DE;Gower LB;Monga M
特发性草酸钙 (CaOx) 肾结石是由于 CaOx 晶体沉积在 Randall 斑块 (RP) 上而形成的。 RP 形成的机制仍不清楚。我们假设 RP 形成类似于血管钙化，涉及细胞外基质成分，包括膜结合囊泡 (MV) 和胶原纤维。为了验证我们的假设，我们严格检查了结石患者的肾乳头组织。对 15 名接受 PCNL 的特发性结石患者进行了 4 mm 肾乳头冷杯活检。立即固定并处理组织，以通过各种光学和电子显微镜技术进行分析。在所有检查的样品中都发现了球状 CaP 晶体，这是 RP 的标志。它们可见于间质以及管状上皮的层压基底膜中。大晶体沉积物由与球晶混合的深色细长链组成。股线显示出类似于胶原蛋白的带状图案。晶体沉积物被胶原纤维和膜结合囊泡包围。能量色散 X 射线微量分析 (EDX) 和电子衍射鉴定出晶体为羟基磷灰石。检查的肾脏数量很少，并且无法获得所有患者的尿液数据。这里给出的结果表明兰德尔斑块中的晶体与胶原蛋白和 MV 相关。胶原纤维出现钙化，囊泡中含有晶体。我们得出的结论是，肾乳头中的晶体沉积可能始于膜囊泡诱导的成核，并通过在胶原蛋白框架内的外围添加晶体而生长。
Idiopathic calcium oxalate (CaOx) kidney stones develop by deposition of CaOx crystals on Randall's plaques (RP). Mechanisms involved in RP formation are still unclear. It is our hypotheses that RP formation is similar to vascular calcification involving components of extracellular matrix including membrane bound vesicles (MV) and collagen fibers. In order to verify our hypothesis we critically examined renal papillary tissue from stone patients. 4 mm cold-cup biopies of renal papillae were performed on fifteen idiopathic stone patients undergoing PCNL. Tissue was immediately fixed and processed for analyses by various light and electron microscopic techniques. Spherulitic CaP crystals, the hallmark of RP's, were seen in all samples examined. They were seen in interstitium as well as laminated basement membrane of tubular epithelia. Large crystalline deposits comprised of dark elongated strands mixed with spherulites. Strands showed banded patterns similar to collagen. Crystal deposits were surrounded by collagen fibers and membrane bound vesicles. Energy dispersive x-ray microanalyses (EDX) and electron diffraction identified the crystals as hydroxyapatite. The number of kidneys examined is small and urinary data was not available for all the patients. Results presented here show that crystals in the Randall's plaques are associated with both the collagen as well as MV. Collagen fibers appeared calcified and vesicles contained crystals. We conclude that crystal deposition in renal papillae may have started with membrane vesicle induced nucleation and grew by addition of crystals on the periphery within a collagen framework.
登录
查看更多内容
影响因子：
--
作者：
Khan SR
通讯作者：
Khan SR
影响因子：
9
作者：
Golub, Ellis E.
通讯作者：
Golub, Ellis E.
影响因子：
4.5
作者：
Miller NL;Gillen DL;Williams JC Jr;Evan AP;Bledsoe SB;Coe FL;Worcester EM;Matlaga BR;Munch LC;Lingeman JE
通讯作者：
Lingeman JE
影响因子：
4.2
作者：
Khan, SR;Maslamani, SA;Hammett-Stabler, C
通讯作者：
Hammett-Stabler, C
石材形成机制的矿物学特征。
影响因子：
--
作者：
Gower LB;Amos FF;Khan SR
通讯作者：
Khan SR