Novel ex-vivo mechanobiological intervertebral disc culture system.
Novel ex-vivo mechanobiological intervertebral disc culture system.
复制标题
新型的前机械生物学椎间盘培养系统。
DOI：
10.1016/j.jbiomech.2011.10.036
复制
发表时间：
2012-01-10
影响因子：
2.4
通讯作者：
Sowa GA
中科院分区：
文献类型：
--
作者：
Hartman RA;Bell KM;Debski RE;Kang JD;Sowa GA
椎间盘退变是导致腰痛的主要原因之一，对社会经济造成了沉重的负担，同时也带来了一系列昂贵的治疗选择。机械负荷在疾病进展和治疗中很重要。连接力学和生物学对于确定负载参数如何影响细胞反应和基质稳态至关重要。开发了一种新的离体实验平台，以促进兔功能脊柱单元（fsu）的原位加载并进行相关的生物学结果测量。该系统设计用于培养箱外的运动，并验证了刚性固定和生理环境条件。使用数字化仪评估相对于新夹具的试样运动；夹具刚度超过试样刚度一个数量级。椎间盘内压力（IDP），使用光纤压力传感器测量，确认刚性和压缩力选择。周围介质控制在37°C， 5% O2/CO2，使用缺氧培养箱的封闭流动回路，并在标本室中使用探针进行验证。fsu在1.0 MPa下进行循环压缩（20个循环）和4小时蠕变。用3-（4,5-二甲基噻唑-2-基）-2,5-二苯基溴化四唑（MTT）分析椎间盘组织的细胞活力，无论负载如何，其活力都很高（约90%）。条件培养基检测ii型胶原降解片段（CTX-II）和与新聚集蛋白合成相关的聚集蛋白表位（CS-846）。CTX-II浓度与负荷无关，但CS-846浓度似乎随负荷而增加。保存完整的FSU允许生理负荷传递和未来的多轴运动以及负荷响应蛋白的鉴定，从而在椎间盘器官培养中形成一个新的生态位。
Intervertebral disc degeneration, a leading cause of low back pain, poses a significant socioeconomic burden with a broad array of costly treatment options. Mechanical loading is important in disease progression and treatment. Connecting mechanics and biology is critical for determining how loading parameters affect cellular response and matrix homeostasis. A novel ex-vivo experimental platform was developed to facilitate in-situ loading of rabbit functional spinal units (FSUs) with relevant biological outcome measures. The system was designed for motion outside of an incubator and validated for rigid fixation and physiologic environmental conditions. Specimen motion relative to novel fixtures was assessed using a digitizer; fixture stiffness exceeded specimen stiffness by an order of magnitude. Intradiscal pressure (IDP), measured using a fiberoptic pressure transducer, confirmed rigidity and compressive force selection. Surrounding media was controlled at 37 °C, 5% O2/CO2 using a closed flow loop with an hypoxic incubator and was validated with probes in the specimen chamber. FSUs were subjected to cyclic compression (20 cycles) and four-hour creep at 1.0 MPa. Disc tissue was analyzed for cell viability using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), which showed high viability (> 90%) regardless of loading. Conditioned media was assayed for type-II collagen degradation fragments (CTX-II) and an aggrecan epitope (CS-846) associated with new aggrecan synthesis. CTX-II concentrations were not associated with loading, but CS-846 concentrations appeared to be increased with loading. Preservation of the full FSU allows physiologic load transmission and future multi-axis motion and identification of load-responsive proteins, thereby forming a new niche in intervertebral disc organ culture.
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影响因子：
2.4
作者：
SHIRAZIADL, A;DROUIN, G
通讯作者：
DROUIN, G
影响因子：
2.4
作者：
van der Veen, Albert J.;Mullender, Margriet G.;Smit, Theo H.
通讯作者：
Smit, Theo H.
影响因子：
1.5
作者：
Csonge, Lajos;Bravo, Daniel;Pellet, Sandor
通讯作者：
Pellet, Sandor
影响因子：
3
作者：
MacLean, JJ;Lee, CR;Iatridis, JC
通讯作者：
Iatridis, JC
DOI：
10.1097/01.bsd.0000092068.78152.00
发表时间：
2004-08-01
影响因子：
--
作者：
Ekström, L;Holm, S;Hansson, T
通讯作者：
Hansson, T