Wide-field time-gated SPAD imager for phasor-based FLIM applications.

Wide-field time-gated SPAD imager for phasor-based FLIM applications.
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DOI:
10.1088/2050-6120/ab6ed7
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发表时间:
2020-02-05
影响因子:
3.2
通讯作者:
Michalet X
Michalet X
中科院分区:
化学3区
文献类型:
--
作者:
Ulku A;Ardelean A;Antolovic M;Weiss S;Charbon E;Bruschini C;Michalet X

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本文描述了一种用于相位flim的新型广域时间门控单光子雪崩二极管(SPAD)阵列的性能,探讨了栅极长度、栅极数和信号强度对测量寿命精度和精度的影响。我们得出的结论是,检测器的功能基本上是一个理想的镜头噪声限制传感器,能够视频速率FLIM测量。在这项工作中使用的相量方法似乎非常适合处理这种类型的非常大的传感器(512 × 512像素)产生的大量数据,即使在少量的门和有限的光子预算的情况下。
We describe the performance of a new wide area time-gated single-photon avalanche diode (SPAD) array for phasor-FLIM, exploring the effect of gate length, gate number and signal intensity on the measured lifetime accuracy and precision. We conclude that the detector functions essentially as an ideal shot noise limited sensor and is capable of video rate FLIM measurement. The phasor approach used in this work appears ideally suited to handle the large amount of data generated by this type of very large sensor (512 × 512 pixels), even in the case of small number of gates and limited photon budget.
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