2D motility tracking of Pseudomonas putida KT2440 in growth phases using video microscopy.
2D motility tracking of Pseudomonas putida KT2440 in growth phases using video microscopy.
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DOI：
10.1016/j.jbiosc.2011.01.007
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发表时间：
2011-05
影响因子：
2.8
通讯作者：
Zhou A
中科院分区：
文献类型：
--
作者：
Davis ML;Mounteer LC;Stevens LK;Miller CD;Zhou A
恶臭假单胞菌KT2440是一种革兰氏阴性游动土壤细菌，在生物修复和生物技术方面具有重要意义。因此，了解它在个体和种群中的运动特征是很重要的。种群特征用改进的Gompertz模型确定。利用视频显微镜和成像软件分析二维(2D)细菌运动轨迹，以量化单个细菌的行为。结果表明，接种密度随接种密度的降低而延长滞后时间，最大比生长速率随接种密度的增加而减小。在整个指数性生长阶段(~20.9微米/秒)，细菌的平均速度基本保持不变，而最大速度在指数性生长阶段的早期以51.2微米/秒的速度达到峰值。恶臭假单胞菌KT2440也喜欢较小的转角，这表明在整个指数生长期鞭毛旋转发生变化后，它们通常会沿着同一方向继续。
Pseudomonas putida KT2440 is a gram negative motile soil bacterium important in bioremediation and biotechnology. Thus, it is important to understand its motility characteristics as individuals and in populations. Population characteristics were determined using a modified Gompertz model. Video microscopy and imaging software were utilized to analyze two dimensional (2D) bacteria movement tracks to quantify individual bacteria behavior. It was determined that inoculum density increased the lag time as seeding densities decreased, and that the maximum specific growth rate decreased as seeding densities increased. Average bacterial velocity remained relatively similar throughout exponential growth phase (~20.9 µm/sec), while maximum velocities peak early in exponential growth phase at a velocity of 51.2 µm/sec. Pseudomonas putida KT2440 also favor smaller turn angles indicating they often continue in the same direction after a change in flagella rotation throughout the exponential growth phase.
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