The immune response to lumpy skin disease virus in cattle is influenced by inoculation route.
The immune response to lumpy skin disease virus in cattle is influenced by inoculation route.
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DOI：
10.3389/fimmu.2022.1051008
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发表时间：
2022
影响因子：
7.3
通讯作者：
中科院分区：
文献类型：
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作者：
肿块性皮肤病病毒 (LSDV) 可引起牛和水牛的严重疾病，并通过食血节肢动物媒介传播。对 LSDV 的适应性和先天免疫反应的详细信息有限，阻碍了控制该疾病的工具的开发。本研究对通过针头接种或节肢动物接种实验性接种 LSDV 的小牛的免疫反应进行了深入分析，其中使用病毒阳性的钙化口螽和埃及伊蚊载体。十七只针头接种的小牛中有七只（41%）出现了以多灶性坏死皮肤结节为特征的临床疾病。相比之下，8/10 (80%) 接种节肢动物的小牛出现临床疾病。从接种后 5 天 (dpi) 起，在针头接种的犊牛中检测到可变的 LSDV 特异性 IFN-γ 免疫反应，临床犊牛（出现皮肤损伤）和非临床犊牛（未出现皮肤损伤）之间没有差异。相比之下，在所有八只临床节肢动物接种的小牛中都检测到了强大且均匀的细胞介导的免疫反应，而在两只非临床节肢动物接种的小牛中检测到的反应很少。 5-7 dpi 后，在所有接种牛中均检测到针对 LSDV 的中和抗体。抗 LSDV IgM 和 IgG 抗体产生的比较显示，临床和非临床针头接种的犊牛之间没有差异，但在非临床节肢动物接种的犊牛中明显存在强烈的 IgM 反应，但在临床节肢动物接种的犊牛中不存在。这表明早期 IgM 的产生与 LSD 的保护相关。这项研究首次提供了临床牛和非临床牛之间免疫反应差异的证据，并强调了在研究 LSD 时使用相关传播模型的重要性。
Lumpy skin disease virus (LSDV) causes severe disease in cattle and water buffalo and is transmitted by hematophagous arthropod vectors. Detailed information of the adaptive and innate immune response to LSDV is limited, hampering the development of tools to control the disease. This study provides an in-depth analysis of the immune responses of calves experimentally inoculated with LSDV via either needle-inoculation or arthropod-inoculation using virus-positive Stomoxys calcitrans and Aedes aegypti vectors. Seven out of seventeen needle-inoculated calves (41%) developed clinical disease characterised by multifocal necrotic cutaneous nodules. In comparison 8/10 (80%) of the arthropod-inoculated calves developed clinical disease. A variable LSDV-specific IFN-γ immune response was detected in the needle-inoculated calves from 5 days post inoculation (dpi) onwards, with no difference between clinical calves (developed cutaneous lesions) and nonclinical calves (did not develop cutaneous lesions). In contrast a robust and uniform cell-mediated immune response was detected in all eight clinical arthropod-inoculated calves, with little response detected in the two nonclinical arthropod-inoculated calves. Neutralising antibodies against LSDV were detected in all inoculated cattle from 5-7 dpi. Comparison of the production of anti-LSDV IgM and IgG antibodies revealed no difference between clinical and nonclinical needle-inoculated calves, however a strong IgM response was evident in the nonclinical arthropod-inoculated calves but absent in the clinical arthropod-inoculated calves. This suggests that early IgM production is a correlate of protection in LSD. This study presents the first evidence of differences in the immune response between clinical and nonclinical cattle and highlights the importance of using a relevant transmission model when studying LSD.
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影响因子：
64.5
作者：
Gilchuk, Iuliia;Gilchuk, Pavlo;Sapparapu, Gopal;Lampley, Rebecca;Singh, Vidisha;Kose, Nurgun;Blum, David L.;Hughes, Laura J.;Satheshkumar, Panayampalli S.;Townsend, Michael B.;Kondas, Ashley V.;Reed, Zachary;Weiner, Zachary;Olson, Victoria A.;Hammarlund, Erika;Raue, Hans-Peter;Slifka, Mark K.;Slaughter, James C.;Graham, Barney S.;Edwards, Kathryn M.;Eisenberg, Roselyn J.;Cohen, Gary H.;Joyce, Sebastian;Crowe, James E., Jr.
通讯作者：
Crowe, James E., Jr.
影响因子：
7.8
作者：
Bamouh Z;Hamdi J;Fellahi S;Khayi S;Jazouli M;Tadlaoui KO;Fihri OF;Tuppurainen E;Elharrak M
通讯作者：
Elharrak M
影响因子：
4.4
作者：
Lefevre EA;Carr BV;Prentice H;Charleston B
通讯作者：
Charleston B
影响因子：
4.2
作者：
KITCHING, RP;BHAT, PP;BLACK, DN
通讯作者：
BLACK, DN
影响因子：
4.3
作者：
Casal, Jordi;Allepuz, Alberto;Beltran-Alcrudo, Daniel
通讯作者：
Beltran-Alcrudo, Daniel