A quantitative assessment of primary and secondary immune responses in cattle using a B cell ELISPOT assay.
A quantitative assessment of primary and secondary immune responses in cattle using a B cell ELISPOT assay.
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DOI：
10.1051/vetres:2008041
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发表时间：
2009-01
影响因子：
4.4
通讯作者：
Charleston B
中科院分区：
文献类型：
--
作者：
Lefevre EA;Carr BV;Prentice H;Charleston B
本研究的目的是全面了解牛模型中Ag特异性浆细胞和记忆B细胞在血流中的外观。为此，我们已经开发了一种方法，允许检测和定量的两种细胞类型的个体小牛免疫与卵清蛋白。在初次应答期间，我们在免疫后第6天和第7天检测到卵白蛋白特异性浆细胞的爆发，随后产生特异性Ab，而记忆B细胞的逐渐增加仅从第15天检测到。正如预期的那样，7周后进行的加强免疫诱导了更快和更强的二次应答。事实上，在第3天和第4天在血液中检测到浆细胞的爆发，随后是Ab滴度的强烈增加。此外，在加强免疫后第5天和第6天检测到记忆B细胞的爆发，而不是逐渐增加。重要的是，我们显示了二次免疫后5个月检测到的抗卵清蛋白特异性IgG滴度与二次免疫后峰值反应时血液中检测到的浆细胞数量之间的强相关性。免疫/接种策略后浆细胞的检测和定量可构成预测Ab应答的幅度和寿命的非常有效的手段。
The aim of the study was to build a comprehensive picture of the appearance in the blood stream of Ag-specific plasma cells and memory B cells in the bovine model. For this purpose, we have developed a method allowing the detection and quantification of both cell types within individual calves immunised with ovalbumin. During the primary response, we detected a burst of ovalbumin-specific plasma cells at days 6 and 7 post-immunisation, which was followed by the production of specific Ab, whereas a gradual increase of memory B cells was only detected from day 15. As expected, a boost immunisation performed 7 weeks later induced a quicker and stronger secondary response. Indeed, a burst of plasma cells was detected in the blood at days 3 and 4, which was followed by a strong increase in Ab titres. Furthermore, a burst of memory B cells, and not a gradual increase, was detected at days 5 and 6 post-boost immunisation. Importantly, we showed a strong correlation between the anti-ovalbumin-specific IgG titres detected 5 months after secondary immunisation and the plasma cell numbers detected in the blood at the peak response after secondary immunisation. The detection and quantification of plasma cells following an immunisation/vaccination strategy could constitute a very effective means for predicting the magnitude and longevity of an Ab response.
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DOI：
10.1016/s0167-5699(00)01687-x
发表时间：
2000-09-01
期刊：
IMMUNOLOGY TODAY
影响因子：
--
作者：
Tarlinton, DM;Smith, KGC
通讯作者：
Smith, KGC
影响因子：
4.4
作者：
Cassese, G;Arce, S;Manz, RA
通讯作者：
Manz, RA
影响因子：
30.5
作者：
Hasbold, J;Corcoran, LM;Hodgkin, PD
通讯作者：
Hodgkin, PD
影响因子：
20.3
作者：
Kelly, Dominic F.;Snape, Mathew D.;Pollard, Andrew J.
通讯作者：
Pollard, Andrew J.
影响因子：
2.8
作者：
COOK, JKA;JONES, BV;CAVANAGH, D
通讯作者：
CAVANAGH, D