Candidatus Sodalis melophagi sp. nov.: phylogenetically independent comparative model to the tsetse fly symbiont Sodalis glossinidius.
Candidatus Sodalis melophagi sp. nov.: phylogenetically independent comparative model to the tsetse fly symbiont Sodalis glossinidius.
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DOI：
10.1371/journal.pone.0040354
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Hypša V
中科院分区：
文献类型：
--
作者：
Chrudimský T;Husník F;Nováková E;Hypša V
苏打属细菌与不同种类的昆虫共生。其中最著名的成员是采采蝇Sodis glossinidius的次生共生体，已成为研究昆虫-细菌共生建立和进化的最重要模式之一。它代表了一种处于向共生过渡的早期/中期状态的细菌，它允许探索一些有趣的主题，如：进入宿主细胞的分泌系统的使用，基因组修饰的节奏，以及与共存的初级共生体的代谢相互作用。在这项研究中，我们描述了一个新种，它可以为采采类共生体提供一个有用的比较模型。它生活在与采采蝇有亲缘关系的绵羊夜蛾中，并在几个重要特征上与绵羊夜蛾相似。与光肩星天牛相似，它与另一种共生细菌--阿森氏菌属的初级共生体共生。嗜黑念珠菌作为一种典型的次生共生菌，侵染包括细菌体在内的多种寄主组织。我们提供了共生体的基本形态和分子特征，并表明这些特征也对应于向共生进化的早期/中期状态。特别是，我们展示了细菌在昆虫细胞培养中以及在无细胞培养基中生活的能力。我们还提供了第三型分泌系统的基本特征，并使用三个参考序列(16个S rDNA，GroEL和spaPQR区域)，我们表明该细菌在苏丹斯属内分支，但独立于先前描述的两个海马科共生体。我们建议将这种新细菌命名为Candidatus Sodalis Melphagi。
Bacteria of the genus Sodalis live in symbiosis with various groups of insects. The best known member of this group, a secondary symbiont of tsetse flies Sodalis glossinidius, has become one of the most important models in investigating establishment and evolution of insect-bacteria symbiosis. It represents a bacterium in the early/intermediate state of the transition towards symbiosis, which allows for exploring such interesting topics as: usage of secretory systems for entering the host cell, tempo of the genome modification, and metabolic interaction with a coexisting primary symbiont. In this study, we describe a new Sodalis species which could provide a useful comparative model to the tsetse symbiont. It lives in association with Melophagus ovinus, an insect related to tsetse flies, and resembles S. glossinidius in several important traits. Similar to S. glossinidius, it cohabits the host with another symbiotic bacterium, the bacteriome-harbored primary symbiont of the genus Arsenophonus. As a typical secondary symbiont, Candidatus Sodalis melophagi infects various host tissues, including bacteriome. We provide basic morphological and molecular characteristics of the symbiont and show that these traits also correspond to the early/intermediate state of the evolution towards symbiosis. Particularly, we demonstrate the ability of the bacterium to live in insect cell culture as well as in cell-free medium. We also provide basic characteristics of type three secretion system and using three reference sequences (16 S rDNA, groEL and spaPQR region) we show that the bacterium branched within the genus Sodalis, but originated independently of the two previously described symbionts of hippoboscoids. We propose the name Candidatus Sodalis melophagi for this new bacterium.
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影响因子：
3.8
作者：
CAMPBELL, BC;BRAGG, TS;TURNER, CE
通讯作者：
TURNER, CE
影响因子：
10.7
作者：
Castresana, J
通讯作者：
Castresana, J
DOI：
10.1073/pnas.182213299
发表时间：
2002-09-17
影响因子：
11.1
作者：
Dale, C;Plague, GR;Moran, NA
通讯作者：
Moran, NA
影响因子：
30.8
作者：
Akman, L;Yamashita, A;Aksoy, S
通讯作者：
Aksoy, S
影响因子：
2.6
作者：
Cheng, Q;Aksoy, S
通讯作者：
Aksoy, S