Molecular epidemiology of respiratory syncytial virus transmission in childcare.
Molecular epidemiology of respiratory syncytial virus transmission in childcare.
复制标题
DOI：
10.1016/j.jcv.2013.04.011
复制
发表时间：
2013-08
影响因子：
8.8
通讯作者：
Englund, Janet A.
中科院分区：
文献类型：
--
作者：
Chu, Helen Y.;Kuypers, Jane;Renaud, Christian;Wald, Anna;Martin, Emily;Fairchok, Mary;Magaret, Amalia;Sarancino, Misty;Englund, Janet A.
呼吸道合胞病毒（RSV）是幼儿严重呼吸道感染的最重要原因。以前没有使用分子技术研究RSV在社区儿童保育环境中的传播。我们试图描述儿童保育中RSV传播的分子流行病学特征，以评估RSV疾病在社区人群中的影响。我们对RSV阳性鼻咽样本进行了测序，这些样本来自一项前瞻性的纵向研究，该研究对三个冬季儿童保育中登记的儿童进行了呼吸道疾病研究。进行系统发育分析以鉴定独特的病毒株。在59例（11%）疾病中检测到RSV。与RSV阴性疾病相比，RSV阳性疾病与更长的症状持续时间和更频繁的医疗保健访问有关。在42例（71%）RSV阳性疾病中检测到另一种呼吸道病毒。RSV病毒载量在有和没有其他呼吸道病毒鉴定的RSV阳性疾病之间没有差异（P = 0.38）。在两个儿童保育室中，50%的儿童在首例病例发生后6天内检测到RSV。对来自一个儿童保育室的13起疾病事件中的5起（38%）进行了测序，结果显示是相同的病毒株，这表明在16天内在房间内快速进行儿童间传播。RSV在儿童保育中迅速传播。鉴于RSV疾病在该人群中的高负担以及RSV在儿童之间的快速传播，儿童保育机构可作为评估新预防策略的理想场所。
Respiratory syncytial virus (RSV) is the most important cause of serious respiratory infections in young children. No prior studies using molecular techniques to examine RSV transmission in the community childcare setting have been performed. We seek to characterize the molecular epidemiology of RSV transmission in childcare to evaluate the impact of RSV disease in a community-based population. We sequenced RSV-positive nasopharyngeal samples from a prospective longitudinal study of respiratory illnesses among children enrolled in childcare during three winter seasons. Phylogenetic analysis was performed to identify unique viral strains. RSV was detected in 59 (11%) illnesses. Compared to RSV-negative illnesses, RSV-positive illnesses were associated with longer symptom duration and increased frequency of health care visits. Another respiratory virus was detected in 42 (71%) RSV-positive illnesses. RSV viral load did not differ between RSV-positive illnesses with and without another respiratory virus identified (P = 0.38). In two childcare rooms, 50% of the children had RSV detected within six days of the first case. Five (38%) of 13 illness episodes from one childcare room were sequenced and shown to be the same viral strain, suggesting rapid child-to-child transmission within the room over a 16 day period. RSV is rapidly transmitted within childcare. Childcare facilities may serve as ideal sites for evaluation of new prevention strategies given the high burden of RSV disease in this population and the rapidity of RSV spread between children.
登录
查看更多内容
DOI：
10.1086/652405
发表时间：
2010-06-01
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Martin ET;Fairchok MP;Kuypers J;Magaret A;Zerr DM;Wald A;Englund JA
通讯作者：
Englund JA
多重与单个病毒呼吸道感染：住院儿童的病毒负荷和临床疾病严重程度。
影响因子：
4.4
作者：
Martin ET;Kuypers J;Wald A;Englund JA
通讯作者：
Englund JA
DOI：
10.1016/j.jcv.2004.03.018
发表时间：
2004-10
期刊：
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
影响因子：
--
作者：
Kuypers J;Wright N;Morrow R
通讯作者：
Morrow R
影响因子：
9.4
作者：
Kuypers, Jane;Wright, Nancy;Morrow, Rhoda
通讯作者：
Morrow, Rhoda
DOI：
10.1056/nejmoa0804877
发表时间：
2009-02-05
期刊：
The New England journal of medicine
影响因子：
--
作者：
Hall CB;Weinberg GA;Iwane MK;Blumkin AK;Edwards KM;Staat MA;Auinger P;Griffin MR;Poehling KA;Erdman D;Grijalva CG;Zhu Y;Szilagyi P
通讯作者：
Szilagyi P