Functional characterization of two defensins, HlDFS1 and HlDFS2, from the hard tick Haemaphysalis longicornis.
Functional characterization of two defensins, HlDFS1 and HlDFS2, from the hard tick Haemaphysalis longicornis.
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长角血蜱硬蜱中两种防御素 HlDFS1 和 HlDFS2 的功能表征
DOI：
10.1186/s13071-017-2397-9
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发表时间：
2017-10-02
影响因子：
3.2
通讯作者：
Dai J
中科院分区：
文献类型：
--
作者：
Sun T;Pan W;Song Y;Zhang J;Wang J;Dai J
背景：蜱是仅次于蚊子的人类节肢动物传播疾病的媒介。蜱主要依靠抗菌肽（AMPs）来抵御微生物，而防御素是蜱固有免疫的主要成分。结果：从中国东南地区采集的长角血蜱cDNA文库中分离到两个新的防御素基因，分别命名为HlDFS1和HlDFS2。这两个基因编码的肽具有2型节肢动物防御素超家族的典型特征。在吸血过程中，这两个基因的表达都有所增加。合成的最小功能肽HlDFS1和HlDFS2对多种革兰氏阳性和革兰氏阴性菌具有广谱抗菌活性。此外，HlDFS1和HlDFS2对一些耐药菌表现出杀菌活性。HlDFS1对白色念珠菌有抑制作用，而HlDFS2对白色念珠菌没有抑制作用。HlDFS1和HlDFS2在低浓度下对人红细胞无明显溶血作用，不影响哺乳动物细胞存活。最后，HlDFS1和HlDFS2对金黄色葡萄球菌和黄体微球菌的致死性感染具有显著的保护作用。结论：HlDFS1和HlDFS2是长角血蜱两种新的功能性防御蛋白。它们对多种革兰氏阳性和革兰氏阴性细菌均有杀菌活性，并能显著保护小鼠免受致死性细菌感染。因此，HlDFS1和HlDFS2可以作为具有抗菌活性的新候选药物引入医学领域。
Background:Ticks are second to mosquitoes as vectors of human arthropod-borne diseases. Ticks rely heavily on antimicrobial peptides (AMPs) to defend against microbes and defensins are major components of innate immunity in ticks.Results:Two novel defensin genes, named HlDFS1 and HlDFS2, were identified from a cDNA library of the hard tick Haemaphysalis longicornis collected in southeast China. The peptides encoded by both genes shares typical features of type-2 arthropod defensin superfamily. The expressions of both genes increased in ticks during blood-feeding. The synthetic minimum functional peptides HlDFS1 and HlDFS2 showed broad spectrum antimicrobial activity against various Gram-positive and Gram-negative bacteria. Moreover, HlDFS1 and HlDFS2 exhibit bactericidal activity to some drug resistant bacteria. HlDFS1, but not HlDFS2, showed inhibitory activity against fungus Candida albicans. HlDFS1 and HlDFS2 had no significant hemolysis effect on human erythrocytes at low concentrations and did not impair mammalian cell survival. Finally, HlDFS1 and HlDFS2 significantly protected mice against lethal infection by Staphylococcus aureus and Micrococcus luteus.Conclusions:HlDFS1 and HlDFS2 are two novel functional defensins from the hard tick Haemaphysalis longicornis. They showed bactericidal activity against various Gram-positive and Gram-negative bacteria and significantly protect mice against lethal bacterial infection. Thus, HlDFS1 and HlDFS2 can be introduced to the medical field as new drug candidates with antibacterial activity.
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影响因子：
3.6
作者：
Alonzo F 3rd;Benson MA;Chen J;Novick RP;Shopsin B;Torres VJ
通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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影响因子：
30.3
作者：
Dai J;Wang P;Adusumilli S;Booth CJ;Narasimhan S;Anguita J;Fikrig E
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Fikrig E
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DOI：
10.1056/nejmoa1010095
发表时间：
2011-04-21
期刊：
The New England journal of medicine
影响因子：
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作者：
Yu XJ;Liang MF;Zhang SY;Liu Y;Li JD;Sun YL;Zhang L;Zhang QF;Popov VL;Li C;Qu J;Li Q;Zhang YP;Hai R;Wu W;Wang Q;Zhan FX;Wang XJ;Kan B;Wang SW;Wan KL;Jing HQ;Lu JX;Yin WW;Zhou H;Guan XH;Liu JF;Bi ZQ;Liu GH;Ren J;Wang H;Zhao Z;Song JD;He JR;Wan T;Zhang JS;Fu XP;Sun LN;Dong XP;Feng ZJ;Yang WZ;Hong T;Zhang Y;Walker DH;Wang Y;Li DX
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