Staphylococcus aureus leucocidin ED contributes to systemic infection by targeting neutrophils and promoting bacterial growth in vivo.
Staphylococcus aureus leucocidin ED contributes to systemic infection by targeting neutrophils and promoting bacterial growth in vivo.
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DOI：
10.1111/j.1365-2958.2011.07942.x
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发表时间：
2012-01
影响因子：
3.6
通讯作者：
Torres VJ
中科院分区：
文献类型：
--
作者：
Alonzo F 3rd;Benson MA;Chen J;Novick RP;Shopsin B;Torres VJ
血液感染金黄色葡萄球菌很常见，可能是致命的。然而，导致金黄色葡萄球菌血液感染致死性的毒力因子还没有明确的定义。我们发现，LukED是一种通常被忽视的白毒素，对小鼠的金黄色葡萄球菌血流感染至关重要。我们还确定LukED通过直接杀死招募到血液种子组织部位的吞噬细胞来促进体内金黄色葡萄球菌的复制。此外，我们确定小鼠中性粒细胞是LukED的主要靶点，因为与LukED突变株相比，野生型金黄色葡萄球菌的毒力更强，这是因为耗尽了中性粒细胞。LukED对小鼠吞噬细胞的体内毒性在金黄色葡萄球菌白毒素中是独一无二的，这意味着它在发病机制中发挥了关键作用。此外，LukED对小鼠吞噬细胞的趋向性突出了小鼠模型在研究LukED病理生物学方面的应用，包括开发和测试抑制毒素活性和控制细菌感染的策略。
Bloodstream infection with Staphylococcus aureus is common and can be fatal. However, virulence factors that contribute to lethality in S. aureus bloodstream infection are poorly defined. We discovered that LukED, a commonly overlooked leukotoxin, is critical for S. aureus bloodstream infection in mice. We also determined that LukED promotes S. aureus replication in vivo by directly killing phagocytes recruited to sites of hematogenously-seeded tissue. Furthermore, we established that murine neutrophils are the primary target of LukED, as the greater virulence of wild type S. aureus compared to a lukED mutant was abrogated by depleting neutrophils. The in vivo toxicity of LukED toward murine phagocytes is unique among S. aureus leukotoxins, implying its crucial role in pathogenesis. Moreover, the tropism of LukED for murine phagocytes highlights the utility of murine models to study LukED pathobiology, including development and testing of strategies to inhibit toxin activity and control bacterial infection.
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DOI：
10.1099/00221287-6-1-2-95
发表时间：
1952-01-01
期刊：
JOURNAL OF GENERAL MICROBIOLOGY
影响因子：
--
作者：
DUTHIE, ES;LORENZ, LL
通讯作者：
LORENZ, LL
影响因子：
3.1
作者：
McNamara, PJ;Bayer, AS
通讯作者：
Bayer, AS
影响因子：
3.7
作者：
Malachowa N;Whitney AR;Kobayashi SD;Sturdevant DE;Kennedy AD;Braughton KR;Shabb DW;Diep BA;Chambers HF;Otto M;DeLeo FR
通讯作者：
DeLeo FR
影响因子：
10.5
作者：
Boisset, Sandrine;Geissmann, Thomas;Romby, Pascale
通讯作者：
Romby, Pascale
影响因子：
3.5
作者：
Gravet, A;Colin, DA;Prévost, G
通讯作者：
Prévost, G