Antibodies against a tick protein, Salp15, protect mice from the Lyme disease agent.
Antibodies against a tick protein, Salp15, protect mice from the Lyme disease agent.
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针对蜱蛋白 Salp15 的抗体可以保护小鼠免受莱姆病病原体的侵害。
DOI：
10.1016/j.chom.2009.10.006
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发表时间：
2009-11-19
影响因子：
30.3
通讯作者：
Fikrig E
中科院分区：
文献类型：
--
作者：
Dai J;Wang P;Adusumilli S;Booth CJ;Narasimhan S;Anguita J;Fikrig E
传统上，疫苗直接针对病原体或微生物毒素。莱姆病由伯氏疏螺旋体引起，是一种由壁虱传播的疾病，目前还没有人类疫苗可用。伯氏杆菌在从媒介传播的过程中结合了扁虱唾液蛋白Salp15，这种相互作用促进了小鼠的感染。我们现在证明，Salp15-抗血清显著保护小鼠免受伯氏杆菌感染。Salp15-抗血清还显著增强了针对伯氏杆菌抗原的抗体的保护能力，如OspA或OspC。用Salp15主动免疫的小鼠也能显著保护小鼠免受壁虱传播的疏螺旋体的感染。体外实验表明，Salp15-抗血清可提高包被Salp15的伯氏杆菌对吞噬细胞的清除能力，提示其作用机制。用微生物感染哺乳动物宿主所需的载体分子进行疫苗接种，为预防莱姆病提供了一种新的策略，这种模式可能适用于许多节肢动物传播的具有重要医学意义的病原体。
Traditionally, vaccines directly target a pathogen or microbial toxin. Lyme disease, caused by Borrelia burgdorferi, is a tick-borne illness for which a human vaccine is not currently available. B. burgdorferi binds a tick salivary protein, Salp15, during transmission from the vector, and this interaction facilitates infection of mice. We now show that Salp15-antiserum significantly protected mice from B. burgdorferi infection. Salp15-antiserum also markedly enhanced the protective capacity of antibodies against B. burgdorferi antigens, such as OspA or OspC. Mice actively immunized with Salp15 were also significantly protected from tick-borne Borrelia. In vitro assays showed that Salp15-antiserum increased the clearance of Salp15-coated B. burgdorferi by phagocytes, suggesting a mechanism of action. Vaccination with a vector molecule that a microbe requires for infection of the mammalian host suggests a new strategy for the prevention of Lyme disease, and this paradigm may be applicable to numerous arthropod-borne pathogens of medical importance.
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影响因子：
3.6
作者：
Eggers, CH;Caimano, MJ;Radolf, JD
通讯作者：
Radolf, JD
DOI：
10.4269/ajtmh.1992.47.605
发表时间：
1992-11-01
影响因子：
3.3
作者：
BARTHOLD, SW;SIDMAN, CL;SMITH, AL
通讯作者：
SMITH, AL
影响因子：
6.7
作者：
Labuda, Milan;Trimnell, Adama R;Lickova, Martina;Kazimirova, Maria;Davies, Gillian M;Lissina, Olga;Hails, Rosie S;Nuttall, Patricia A
通讯作者：
Nuttall, Patricia A
影响因子：
2.5
作者：
de la Fuente, Jose;Almazan, Consuelo;Willadsen, Peter
通讯作者：
Willadsen, Peter
影响因子：
3.1
作者：
de Silva, AM;Zeidner, NS;Fikrig, E
通讯作者：
Fikrig, E