Ultrafast Tracking of Oxygen Dynamics During Proton FLASH.
Ultrafast Tracking of Oxygen Dynamics During Proton FLASH.
复制标题
质子闪光过程中氧动力学的超快跟踪。
DOI：
10.1016/j.ijrobp.2022.03.016
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发表时间：
2022-07-01
影响因子：
7
通讯作者：
Vinogradov, Sergei A.
中科院分区：
文献类型：
--
作者：
El Khatib, Mirna;van Slyke, Alexander L.;Velalopoulou, Anastasia;Kim, Michele M.;Shoniyozov, Khayrullo;Allu, Srinivasa Rao;Diffenderfer, Eric E.;Busch, Theresa M.;Wiersma, Rodney D.;Koch, Cameron J.;Vinogradov, Sergei A.
以超快剂量率递送的放射治疗（称为FLASH RT）已被证明通过选择性地保护正常组织而提供与常规放射治疗相比的治疗优势。氧的放射化学消耗已被提出来支持FLASH效应，然而，这种假设的实验验证一直缺乏，部分原因是由于无法测量氧合率与FLASH兼容。我们提出了一种新的变体的磷光淬灭方法跟踪氧动力学的速率达到~3.3 kHz。使用可溶性Oxyphor探针，我们能够解决，在体外和体内，氧动力学在质子FLASH的交付时间。(1)在体外，在含有牛血清白蛋白（BSA）的溶液中，常规辐照（在75 μM [O2]时约为13%）的O2消耗g值（每辐射剂量消耗的O2摩尔/L，例如μM/戈伊）高于FLASH，在低氧区（<25 μM [O2]），它们随氧浓度降低。(2)在体内，通过单个FLASH的氧消耗不足以在最初氧合良好的组织中实现严重缺氧;以及（3）测量的g值似乎与基线氧水平相关。所开发的方法应有助于放射生物学研究，如旨在揭示FLASH效应机制的研究。FLASH效应可能部分源于常规与FLASH RT的耗氧量g值的氧依赖性差异。
Radiation therapy delivered at ultrafast dose rates, known as FLASH RT, has been shown to provide a therapeutic advantage compared to conventional radiotherapy by selectively protecting normal tissues. Radiochemical depletion of oxygen has been proposed to underpin the FLASH effect, however experimental validation of this hypotheses has been lacking, in part due to the inability to measure oxygenation at rates compatible with FLASH. We present a new variant of the phosphorescence quenching method for tracking oxygen dynamics with rates reaching up to ~3.3 kHz. Using soluble Oxyphor probes we were able to resolve, both in vitro and in vivo, oxygen dynamics during the time of delivery of proton FLASH. (1) In vitro in solutions containing bovine serum albumin (BSA) the O2 depletion g-values (moles/L of O2 depleted per radiation dose, e.g. μM/Gy) are higher for conventional irradiation (by ~13% at 75 μM [O2]) than for FLASH, and in the low-oxygen region (<25 μM [O2]) they decrease with oxygen concentration. (2) In vivo, depletion of oxygen by a single FLASH is insufficient to achieve severe hypoxia in initially well-oxygenated tissue; and (3) the g-values measured appear to correlate with baseline oxygen levels. The developed method should be instrumental in radiobiological studies, such as studies aimed at unraveling the mechanism of the FLASH effect. The FLASH effect could in part originate from the difference in the oxygen dependencies of the oxygen consumption g-values for conventional vs FLASH RT.
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影响因子：
3.8
作者：
Favaudon V;Labarbe R;Limoli CL
通讯作者：
Limoli CL
DOI：
10.1016/j.ijrobp.2021.03.056
发表时间：
2021-09-01
期刊：
International journal of radiation oncology, biology, physics
影响因子：
--
作者：
Cao X;Zhang R;Esipova TV;Allu SR;Ashraf R;Rahman M;Gunn JR;Bruza P;Gladstone DJ;Williams BB;Swartz HM;Hoopes PJ;Vinogradov SA;Pogue BW
通讯作者：
Pogue BW
影响因子：
3.4
作者：
LING, CC;MICHAELS, HB;PETERSON, EC
通讯作者：
PETERSON, EC
影响因子：
3.4
作者：
MICHAELS, HB;EPP, ER;PETERSON, EC
通讯作者：
PETERSON, EC
影响因子：
5.6
作者：
Hughes JR;Parsons JL
通讯作者：
Parsons JL