Al2O3 doped apatite-wollastonite containing glass ceramic provokes osteogenic differentiation of marrow stromal stem cells.
Al2O3 doped apatite-wollastonite containing glass ceramic provokes osteogenic differentiation of marrow stromal stem cells.
复制标题
含有 Al2O3 掺杂磷灰石-硅灰石的玻璃陶瓷可激发骨髓基质干细胞的成骨分化。
DOI：
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
K. Okunaga
中科院分区：
文献类型：
--
作者：
H. Ohgushi;T. Yoshikawa;H. Nakajima;S. Tamai;Y. Dohi;K. Okunaga
Fresh marrow cells were obtained from femora of Fischer rats and cultured in a medium containing 15% fetal calf serum (FCS) until confluence. After trypsinization, cells were subcultured at a cell density of 100 x 10(3)/35-mm well in the presence of FCS, beta-glycerophosphate, and ascorbic acid phosphate on four different culture substrata. The period of subculture was 2 weeks; the substrata used were the culture dish, apatite-wollastonite containing glass ceramic (AW), hydroxyapatite coated AW (HA/AW), and Al2O3 doped AW (Al/AW). The HA coating was attained by the incubation of AW in simulated physiological solution. The glass matrix of AW and HA/AW contained MgO, CaO, P2O5, and SiO2; Al/AW contained Al2O3 in addition to these components. The subculture on Al/AW substratum showed many alkaline phosphatase (ALP) positive nodules and the highest ALP activity. On a Northern blot analysis the housekeeping gene of beta-actin mRNA was evenly detected from the cells cultured on all substrata; however, bone-specific osteocalcin mRNA was only detected from the cells on Al/AW. These results indicate that Al/AW provokes the osteoblastic differentiation of marrow stromal stem cells.
影响因子：
2.7
作者：
RICKARD, DJ;SULLIVAN, TA;KAZHDAN, I
通讯作者：
KAZHDAN, I
DOI：
10.1016/s0021-9258(19)69522-0
发表时间：
1981-04
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
P. Price;J. W. Lothringer;S. Baukol;A. Reddi
通讯作者：
P. Price;J. W. Lothringer;S. Baukol;A. Reddi
影响因子：
15.9
作者：
PRICE, PA;PARTHEMORE, JG;NISHIMOTO, SK
通讯作者：
NISHIMOTO, SK