Dual roles for the telomeric repeats in chromosomally integrated human herpesvirus-6.
Dual roles for the telomeric repeats in chromosomally integrated human herpesvirus-6.
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DOI：
10.1038/srep04559
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发表时间：
2014-04-02
影响因子：
4.6
通讯作者：
Kurahashi H
中科院分区：
文献类型：
--
作者：
Ohye T;Inagaki H;Ihira M;Higashimoto Y;Kato K;Oikawa J;Yagasaki H;Niizuma T;Takahashi Y;Kojima S;Yoshikawa T;Kurahashi H
大约1%的健康人在宿主染色体中携带人类疱疹病毒-6。这被称为染色体整合疱疹病毒-6(CIHHV-6)。在这项研究中，我们调查了六个携带CIHHV-6B的个体的染色体整合位点。利用FISH，我们发现HHV-6B信号一直位于端粒区域。在所有病例中，整合病毒的近端终点都被定位在DR-R内的两个端粒重复序列(TRSS)之一。在两个案例中，我们分离了病毒TRS和人类端粒重复序列之间的连接片段。所有病例的远端终点均在远端TRS处标测。远端转录因子的大小约为5 kb，足以完成细胞端粒功能。我们的结论是，病毒DR区的TRs对CIHHV-6具有双重功能：同源介导的整合到染色体的端粒区域和形成新的端粒，然后稳定地传播。
Approximately 1 percent of healthy individuals carry human herpesvirus-6 within a host chromosome. This is referred to as chromosomally integrated herpesvirus-6 (CIHHV-6). In this study, we investigated the chromosomal integration site in six individuals harboring CIHHV-6B. Using FISH, we found that HHV-6B signals are consistently located at the telomeric region. The proximal endpoints of the integrated virus were mapped at one of two telomere-repeat-like sequences (TRSs) within the DR-R in all cases. In two cases, we isolated junction fragments between the viral TRS and human telomere repeats. The distal endpoints were mapped at the distal TRS in all cases. The size of the distal TRS was found to be ~5 kb which is sufficient to fulfill cellular telomeric functions. We conclude that the viral TRS in the DR regions fulfill dual functions for CIHHV-6: homology-mediated integration into the telomeric region of the chromosome and neo-telomere formation that is then stably transmitted.
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影响因子：
3.1
作者：
Achour, Abla;Malet, Isabelle;Agut, Henri
通讯作者：
Agut, Henri
DOI：
10.1126/science.1170633
发表时间：
2009-11-13
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
de Lange T
通讯作者：
de Lange T
影响因子：
64.8
作者：
Linardopoulou, EV;Williams, EM;Trask, BJ
通讯作者：
Trask, BJ
影响因子：
5.4
作者：
Isegawa, Y;Mukai, T;Yamanishi, K
通讯作者：
Yamanishi, K
影响因子：
3.8
作者：
KONDO, K;KONDO, T;YAMANISHI, K
通讯作者：
YAMANISHI, K