Magnetic particle templating of hydrogels: engineering naturally derived hydrogel scaffolds with 3D aligned microarchitecture for nerve repair.
Magnetic particle templating of hydrogels: engineering naturally derived hydrogel scaffolds with 3D aligned microarchitecture for nerve repair.
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DOI：
10.1088/1741-2552/ab4a22
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发表时间：
2020-02-12
影响因子：
4
通讯作者：
Schmidt CE
中科院分区：
文献类型：
--
作者：
Lacko CS;Singh I;Wall MA;Garcia AR;Porvasnik SL;Rinaldi C;Schmidt CE
然而，水凝胶支架在组织工程应用中有着广泛的应用前景，但通常缺乏组织模拟结构。因此，在这项工作中，我们试图开发一种新的技术，用于将自下而上设计的水凝胶支架内的对齐管状结构并入。我们报告了一种平台制造技术-磁性模板-与其他方法不同的是，它使用可溶性磁性藻酸盐微粒（MAMs）在施加的磁场下形成对齐的柱状结构。去除MAMs产生具有对齐的管状微结构的支架，其可以促进用于各种应用的细胞重塑。这种方法提供了先进的应用微结构直径和生物改性的控制。在这里，我们试图复制天然神经基板的微结构，使用磁性模板的水凝胶组成的甲基丙烯酸缩水甘油酯透明质酸和胶原蛋白I。磁性模板水凝胶的特点是颗粒排列和微孔。通过96.8%的氧化铁纳米颗粒的去除来验证总体MAM去除功效。压缩力学性能良好匹配的周围神经组织在0.93 kPa和1.29 kPa，分别。在体外，模板化水凝胶在12小时内表现出约36%的降解速度，并被发现引导轴突从背根神经节延伸。最后，在利用10 mm大鼠坐骨神经缺损模型的初步体内研究中，与非模板化对照相比，磁性模板化水凝胶表现出有希望的结果，具有定性增加的重塑和轴突再生。这种简单且可扩展的技术具有在长的长度尺度上控制管状微结构的灵活性，并且因此具有满足用于组织再生的工程支架（包括神经引导支架）的需要的潜力。
Hydrogel scaffolds hold promise for a myriad of tissue engineering applications, however, often lack tissue-mimetic architecture. Therefore, in this work, we sought to develop a new technology for the incorporation of aligned tubular architecture within hydrogel scaffolds engineered from the bottom-up. We report a platform fabrication technology -- magnetic templating-- distinct from other approaches in that it uses dissolvable magnetic alginate microparticles (MAMs) to form aligned columnar structures under an applied magnetic field. Removal of the MAMs yields scaffolds with aligned tubular microarchitecture that can promote cell remodeling for a variety of applications. This approach affords control of microstructure diameter and biological modification for advanced applications. Here, we sought to replicate the microarchitecture of the native nerve basal lamina using magnetic templating of hydrogels composed of glycidyl methacrylate hyaluronic acid and collagen I. Magnetically templated hydrogels were characterized for particle alignment and micro-porosity. Overall MAM removal efficacy was verified by 96.8% removal of iron oxide nanoparticles. Compressive mechanical properties were well-matched to peripheral nerve tissue at 0.93 kPa and 1.29 kPa, respectively. In vitro, templated hydrogels exhibited approximately 36% faster degradation over 12 hours, and were found to guide axon extension from dorsal root ganglia. Finally, in a pilot in vivo study utilizing a 10-mm rat sciatic nerve defect model, magnetically templated hydrogels demonstrated promising results with qualitatively increased remodeling and axon regeneration compared to non-templated controls. This simple and scalable technology has the flexibility to control tubular microstructure over long length scales, and thus the potential to meet the need for engineered scaffolds for tissue regeneration, including nerve guidance scaffolds.
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影响因子：
4.1
作者：
Hu, Chengzhi;Uchida, Tomoyuki;Kwon, Guiryong
通讯作者：
Kwon, Guiryong
影响因子：
2.5
作者：
Haessler, Ulrike;Teo, Jeremy C. M.;Swartz, Melody A.
通讯作者：
Swartz, Melody A.
影响因子：
10.8
作者：
Rose JC;Cámara-Torres M;Rahimi K;Köhler J;Möller M;De Laporte L
通讯作者：
De Laporte L
影响因子：
14
作者：
Scott, John B.;Afshari, Mehdi;Saul, Justin M.
通讯作者：
Saul, Justin M.
影响因子：
14
作者：
Kim, Young-tae;Haftel, Valerie K.;Bellamkonda, Ravi V.
通讯作者：
Bellamkonda, Ravi V.