Signaling role of fructose mediated by FINS1/FBP in Arabidopsis thaliana.
Signaling role of fructose mediated by FINS1/FBP in Arabidopsis thaliana.
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FINS1/FBP在拟南芥中介导的果糖的信号传导作用。
DOI：
10.1371/journal.pgen.1001263
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发表时间：
2011-01-06
期刊：
影响因子：
4.5
通讯作者：
Yoo SD
中科院分区：
文献类型：
--
作者：
Cho YH;Yoo SD
糖是进化上保守的信号分子，调节单细胞和多细胞生物的生长和发育。作为产糖光合生物，植物利用葡萄糖作为其主要信号分子之一。然而，由于控制植物生理和发育程序的代谢物和激素相互作用的复杂性，其他糖信号分子及其调节因子的细节仍然难以捉摸。我们结合了功能获得性细胞筛选和功能丧失性反向遗传分析的信息，证明果糖在拟南芥中是一种信号分子。果糖信号诱导幼苗发育停滞，并以类似于葡萄糖的方式与植物胁迫激素信号相互作用。对于果糖信号转导反应，植物葡萄糖传感器己激酶1（HXK 1）是不敏感的，而果糖不敏感1（FINS 1），一种假定的果糖-1，6-二磷酸酶，起着至关重要的作用。有趣的是，FINS 1在果糖信号传导中的功能似乎与其在糖代谢中的催化活性无关。遗传分析进一步表明，FINS 1依赖的果糖信号可能作用于脱落酸途径的下游，尽管事实上HXK 1依赖的葡萄糖信号在激素合成的上游起作用。我们的研究结果表明，果糖，葡萄糖和脱落酸的多层控制微调植物自养过渡和调节种子萌发后早期幼苗的建立。在众多的植物糖代谢产物中，葡萄糖信号转导受到了最多的关注。虽然果糖也是一种丰富的己糖，但其在植物生长和发育中的信号作用迄今尚未得到明确和系统的解决。我们发现，果糖作为一种调节糖代谢产物，与植物激素脱落酸（阿坝）和乙烯信号相互作用。thaliana.果糖依赖性生长反应由果糖不敏感1（FINS 1）介导，FINS 1编码一种古老的代谢酶，即假定的果糖-1，6-二磷酸酶。有趣的是，FINS 1在蔗糖生物合成中的催化功能与其在果糖信号传导中的调节作用无关。FINS 1似乎在参与阿坝合成的葡萄糖不敏感1的下游起作用。综上所述，虽然果糖和葡萄糖具有独特的调控途径，但它们也与植物逆境和防御激素有着共同的信号相互作用，并协调A. thaliana.果糖影响哺乳动物的细胞信号传导，并引起各种代谢综合征。然而，果糖和生理疾病之间的直接关系尚未建立。由于FINS 1在进化上是保守的，我们对其信号功能的遗传评估可能会提供有关动物和植物中果糖信号的有用信息。
Sugars are evolutionarily conserved signaling molecules that regulate the growth and development of both unicellular and multicellular organisms. As sugar-producing photosynthetic organisms, plants utilize glucose as one of their major signaling molecules. However, the details of other sugar signaling molecules and their regulatory factors have remained elusive, due to the complexity of the metabolite and hormone interactions that control physiological and developmental programs in plants. We combined information from a gain-of-function cell-based screen and a loss-of-function reverse-genetic analysis to demonstrate that fructose acts as a signaling molecule in Arabidopsis thaliana. Fructose signaling induced seedling developmental arrest and interacted with plant stress hormone signaling in a manner similar to that of glucose. For fructose signaling responses, the plant glucose sensor HEXOKINASE1 (HXK1) was dispensable, while FRUCTOSE INSENSITIVE1 (FINS1), a putative FRUCTOSE-1,6-BISPHOSPHATASE, played a crucial role. Interestingly, FINS1 function in fructose signaling appeared to be independent of its catalytic activity in sugar metabolism. Genetic analysis further indicated that FINS1–dependent fructose signaling may act downstream of the abscisic acid pathway, in spite of the fact that HXK1–dependent glucose signaling works upstream of hormone synthesis. Our findings revealed that multiple layers of controls by fructose, glucose, and abscisic acid finely tune the plant autotrophic transition and modulate early seedling establishment after seed germination. Among the many plant sugar metabolites, glucose signaling has received the most attention. Although fructose is also an abundant hexose, its signaling role in plant growth and development has not been addressed clearly and systematically to date. We found that fructose functions as a regulatory sugar metabolite and interacts with signaling by the plant hormones abscisic acid (ABA) and ethylene in A. thaliana. The fructose-dependent growth response is mediated by FRUCTOSE INSENSITIVE1 (FINS1), which encodes an ancient metabolic enzyme, putative fructose-1,6-bisphosphatase. Interestingly, the catalytic function of FINS1 in sucrose biosynthesis is dispensable for its regulatory role in fructose signaling. FINS1 appears to act downstream of GLUCOSE-INSENSITIVE1, which is involved in ABA synthesis. Overall, it is evident that although fructose and glucose have unique regulatory pathways, they also share some signaling interactions with plant stress and defense hormones and coordinate early seedling establishment of A. thaliana. Fructose affects cell signaling in mammals and causes various metabolic syndromes. However, a direct relationship between fructose and physiological diseases has not been established yet. Because FINS1 is evolutionarily conserved, our genetic evaluation of its signaling function may provide useful information about fructose signaling in animals as well as plants.
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影响因子：
7.2
作者：
German, MA;Dai, N;Granot, D
通讯作者：
Granot, D
影响因子：
5.2
作者：
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DOI：
10.1073/pnas.95.8.4784
发表时间：
1998-04-14
影响因子：
11.1
作者：
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通讯作者：
Bush, DR
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7.4
作者：
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影响因子：
11.6
作者：
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