MiR-92b-3p Inhibits Proliferation of HER2-Positive Breast Cancer Cell by Targeting circCDYL.
MiR-92b-3p Inhibits Proliferation of HER2-Positive Breast Cancer Cell by Targeting circCDYL.
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MiR-92b-3p 通过靶向 circCDYL 抑制 HER2 阳性乳腺癌细胞的增殖
DOI：
10.3389/fcell.2021.707049
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发表时间：
2021
影响因子：
5.5
通讯作者：
Gong C
中科院分区：
文献类型：
--
作者：
Liang G;Ling Y;Lin Q;Shi Y;Luo Q;Cen Y;Mehrpour M;Hamai A;Li J;Gong C
目的环状RNA（circular RNA，circRNA）是一类新发现的RNA，在调节多种肿瘤细胞命运方面具有重要的生物学功能。之前，我们已经证明了circRNA circCDYL的过表达通过miR-1275-ULK 1/ATG 7-自噬轴促进HER 2阴性（HER 2-）乳腺癌的进展。然而，circCDYL在HER 2阳性（HER 2+）乳腺癌中的作用，特别是其在调节细胞增殖（细胞命运的最重要特征之一）中的作用尚不清楚。材料与方法采用qRT-PCR和原位杂交技术检测乳腺癌组织或细胞系中circCDYL和miR-92 b-3 p的表达。通过平板集落形成和细胞活力测定以及原位动物模型评估circCDYL和miR-92 b-3 p的生物学功能。在机制研究中，进行了circRNAs pull-down、RNA免疫沉淀、双荧光素酶报告、western blot、免疫组织化学和免疫荧光染色测定。结果CircCDYL在HER 2+乳腺癌组织中呈高表达，与HER 2-乳腺癌组织相似。沉默HER 2基因对HER 2+乳腺癌细胞中circCDYL的表达没有影响。circCDYL的过表达促进HER 2+乳腺癌细胞的增殖，但不是通过miR-1275-ULK 1/ATG 7-自噬轴。CircRNA pull down和miRNA深度测序证实了miR-92 b-3 p和circCDYL的结合。有趣的是，circCDYL不充当miR-92 b-3 p海绵，而是以miR-92 b-3 p依赖性沉默方式降解。临床上，circCDYL和miR-92 b-3 p的表达与HER 2+乳腺癌患者的临床结局相关。结论miR-92 b-3 p依赖的cyclCDYL切割是HER 2+乳腺癌细胞增殖调控的重要机制。CircCDYL被证明是HER 2+乳腺癌的潜在治疗靶点，circCDYL和miR-92 b-3 p可能是预测HER 2+乳腺癌患者临床结局的潜在生物标志物。
Objectives Circular RNA (circRNA) is a novel class of RNA, which exhibits powerful biological function in regulating cellular fate of various tumors. Previously, we had demonstrated that over-expression of circRNA circCDYL promoted progression of HER2-negative (HER2–) breast cancer via miR-1275-ULK1/ATG7-autophagic axis. However, the role of circCDYL in HER2-positive (HER2+) breast cancer, in particular its role in modulating cell proliferation, one of the most important characteristics of cellular fate, is unclear. Materials and methods qRT-PCR and in situ hybridization analyses were performed to examine the expression of circCDYL and miR-92b-3p in breast cancer tissues or cell lines. The biological function of circCDYL and miR-92b-3p were assessed by plate colony formation and cell viability assays and orthotopic animal models. In mechanistic study, circRNAs pull-down, RNA immunoprecipitation, dual luciferase report, western blot, immunohistochemical and immunofluorescence staining assays were performed. Results CircCDYL was high-expressed in HER2+ breast cancer tissue, similar with that in HER2– breast cancer tissue. Silencing HER2 gene had no effect on expression of circCDYL in HER2+ breast cancer cells. Over-expression of circCDYL promoted proliferation of HER2+ breast cancer cells but not through miR-1275-ULK1/ATG7-autophagic axis. CircRNA pull down and miRNA deep-sequencing demonstrated the binding of miR-92b-3p and circCDYL. Interestingly, circCDYL did not act as miR-92b-3p sponge, but was degraded in miR-92b-3p-dependent silencing manner. Clinically, expression of circCDYL and miR-92b-3p was associated with clinical outcome of HER2+ breast cancer patients. Conclusion MiR-92b-3p-dependent cleavage of circCDYL was an essential mechanism in regulating cell proliferation of HER2+ breast cancer cells. CircCDYL was proved to be a potential therapeutic target for HER2+ breast cancer, and both circCDYL and miR-92b-3p might be potential biomarkers in predicting clinical outcome of HER2+ breast cancer patients.
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影响因子：
4.5
作者：
Marzec M
通讯作者：
Marzec M
FBXW7 环状 RNA 在抑制神经胶质瘤肿瘤发生中的新作用。
DOI：
10.1093/jnci/djx166
发表时间：
2018-03-01
期刊：
Journal of the National Cancer Institute
影响因子：
--
作者：
Yang Y;Gao X;Zhang M;Yan S;Sun C;Xiao F;Huang N;Yang X;Zhao K;Zhou H;Huang S;Xie B;Zhang N
通讯作者：
Zhang N
影响因子：
3.9
作者：
Galvão-Lima LJ;Morais AHF;Valentim RAM;Barreto EJSS
通讯作者：
Barreto EJSS
MicroRNA-92B-3P的表达改变预测了前列腺癌患者的生存结果，并在肿瘤进展中起癌基因的作用。
影响因子：
2.9
作者：
Wang G;Cheng B;Jia R;Tan B;Liu W
通讯作者：
Liu W
自噬相关的 circRNA circCDYL 增强自噬并促进乳腺癌进展
影响因子：
37.3
作者：
Liang, Gehao;Ling, Yun;Gong, Chang
通讯作者：
Gong, Chang