Chronic Stress Alters Astrocyte Morphology in Mouse Prefrontal Cortex.
Chronic Stress Alters Astrocyte Morphology in Mouse Prefrontal Cortex.
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DOI：
10.1093/ijnp/pyab052
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发表时间：
2021-10-23
期刊：
影响因子：
--
通讯作者：
Banasr M
中科院分区：
文献类型：
--
作者：
Codeluppi SA;Chatterjee D;Prevot TD;Bansal Y;Misquitta KA;Sibille E;Banasr M
在应激相关疾病患者和啮齿动物应激模型中，前额叶皮层的神经形态变化一直被报道，但应激对星形胶质细胞形态的影响及其与行为缺陷的潜在联系相对未知。为了回答这些问题，在胶质原纤维酸蛋白（GFAP）启动子下表达绿色荧光蛋白（GFP）的转基因小鼠受到7、21或35天的慢性抑制应激（CRS）。crs诱导的行为对快感缺乏症和焦虑样行为的影响分别使用蔗糖摄入量和表型测试进行测量。使用Sholl分析评估前额皮质GFP+或GFAP+细胞形态，并使用相关分析确定与行为的关联。暴露于crs的雄性和雌性小鼠在第7、21和35天表现出焦虑样行为，在第35天表现出快感缺乏样行为。GFAP+细胞形态学分析显示，CRS治疗21天和35天后，远端突起明显萎缩。CRS诱导GFP+细胞远端桡骨交点减少，并伴有近端突起增加。在雄性中，最远桡骨步的交叉点数量与快感缺失样行为显著相关(r = 0.622, P <。对GFP+细胞和行为情绪进行z评分计算所有行为测量缺陷（r = - 0.667, P < 0.05）。在女性中观察到类似但不显著的相关性。GFP+细胞萎缩与焦虑样行为无相关性。慢性应激暴露诱导皮层星形胶质细胞进行性萎缩，可能导致与应激相关疾病相关的神经可塑性不良和行为改变。
Neuromorphological changes are consistently reported in the prefrontal cortex of patients with stress-related disorders and in rodent stress models, but the effects of stress on astrocyte morphology and the potential link to behavioral deficits are relatively unknown. To answer these questions, transgenic mice expressing green fluorescent protein (GFP) under the glial fibrillary acid protein (GFAP) promotor were subjected to 7, 21, or 35 days of chronic restraint stress (CRS). CRS-induced behavioral effects on anhedonia- and anxiety-like behaviors were measured using the sucrose intake and the PhenoTyper tests, respectively. Prefrontal cortex GFP+ or GFAP+ cell morphology was assessed using Sholl analysis, and associations with behavior were determined using correlation analysis. CRS-exposed male and female mice displayed anxiety-like behavior at 7, 21, and 35 days and anhedonia-like behavior at 35 days. Analysis of GFAP+ cell morphology revealed significant atrophy of distal processes following 21 and 35 days of CRS. CRS induced similar decreases in intersections at distal radii for GFP+ cells accompanied by increased proximal processes. In males, the number of intersections at the most distal radius step significantly correlated with anhedonia-like behavior (r = 0.622, P < .05) for GFP+ cells and with behavioral emotionality calculated by z-scoring all behavioral measured deficits (r = −0.667, P < .05). Similar but not significant correlations were observed in females. No correlation between GFP+ cell atrophy with anxiety-like behavior was found. Chronic stress exposure induces a progressive atrophy of cortical astroglial cells, potentially contributing to maladaptive neuroplastic and behavioral changes associated with stress-related disorders.
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影响因子：
3.3
作者：
Huang, Niannian;Yang, Chun;Xu, Hui
通讯作者：
Xu, Hui
影响因子：
3.3
作者：
Fee, Corey;Prevot, Thomas;Sibille, Etienne
通讯作者：
Sibille, Etienne
影响因子：
16.2
作者：
Grienberger, Christine;Konnerth, Arthur
通讯作者：
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关于精神疾病的新看法：超越胶水的神经胶质参与。
影响因子：
5.3
作者：
Elsayed M;Magistretti PJ
通讯作者：
Magistretti PJ
影响因子：
10.6
作者：
Bailey, Craig D. C.;Alves, Nyresa C.;Lambe, Evelyn K.
通讯作者：
Lambe, Evelyn K.