Chromosome-scale assembly of the Monopterus genome.
Chromosome-scale assembly of the Monopterus genome.
复制标题
黄鳝基因组的染色体规模组装
DOI：
10.1093/gigascience/giy046
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发表时间：
2018-05-01
期刊：
影响因子：
9.2
通讯作者：
Zhou R
中科院分区：
文献类型：
--
作者：
Zhao X;Luo M;Li Z;Zhong P;Cheng Y;Lai F;Wang X;Min J;Bai M;Yang Y;Cheng H;Zhou R
摘要背景硬骨鱼因其自然的性别转换和较小的基因组，以及巨大的经济和潜在的医学价值，正在成为一种新的生物学研究模式。然而，目前还没有关于黄龙的基因组信息。在这里，我们对白色分枝杆菌的基因组进行了测序和重新组装，并报道了保守共轭(CAFS)辅助的FISH步行组装新染色体的方法。利用CAF将32 8个支架组装成12条染色体，覆盖了555Mb的基因组序列，占支架组装序列(∼689Mb)的81.3%。共定位18个 ，660个基因定位在染色体上，呈非随机分布。结论首次报道了黄鳝的参考基因组，并提供了一种有效的染色体水平组装的CAFS策略，这不仅为进一步研究遗传、进化和发育，特别是性别决定提供了宝贵的资源，也为该物种的品种改良提供了一种有效的CAFS策略。
Abstract Background The teleost fish Monopterus albus is emerging as a new model for biological studies due to its natural sex transition and small genome, in addition to its enormous economic and potential medical value. However, no genomic information for the Monopterus is currently available. Findings Here, we sequenced and de novo assembled the genome of M. albus and report the de novochromosome assembly by FISH walking assisted by conserved synteny (Cafs). Using Cafs, 328 scaffolds were assembled into 12 chromosomes, which covered genomic sequences of 555 Mb, accounting for 81.3% of the sequences assembled in scaffolds (∼689 Mb). A total of 18 ,660 genes were mapped on the chromosomes and showed a nonrandom distribution along chromosomes. Conclusions We report the first reference genome of the Monopterus and provide an efficient Cafs strategy for a de novo chromosome-level assembly of the Monopterus genome, which provides a valuable resource, not only for further studies in genetics, evolution, and development, particularly sex determination, but also for breed improvement of the species.
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影响因子：
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通讯作者：
Eichler EE