Mesenchymal progenitor cells derived from traumatized muscle enhance neurite growth.
Mesenchymal progenitor cells derived from traumatized muscle enhance neurite growth.
复制标题
DOI：
10.1002/term.539
复制
发表时间：
2013-06
影响因子：
3.3
通讯作者：
Tuan, Rocky S.
中科院分区：
文献类型：
--
作者：
Jackson, Wesley M.;Alexander, Peter G.;Bulken-Hoover, Jamie D.;Vogler, Jared A.;Ji, Youngmi;McKay, Patricia;Nesti, Leon J.;Tuan, Rocky S.
关键词：
周围神经再生的成功取决于损伤区域轴突桥接和髓鞘形成的速度和质量。神经突生长和雪旺细胞的迁移受神经再生时产生的神经营养因子的调节，这些过程可以通过间充质干细胞（MSC）来增强，间充质干细胞也产生神经营养因子和其他改善功能性组织再生的因子。我们的实验室最近已经确定了一个人口的间充质祖细胞（MPC），可以收获创伤肌肉组织清创和收集在骨科重建手术。本研究的目的是确定创伤后肌肉来源的MPCs是否表现出与骨髓来源的MSC相当的神经营养功能。相似的基因和蛋白质水平表达的特定的神经营养因子，观察两种细胞类型，我们本地化的神经源性细胞内细胞标志物（脑源性神经营养因子和巢蛋白）的亚群的MPC和MSC。此外，我们证明了MPC分泌的因子足以增强鸡胚背根神经节（DRG）模型中的体外轴突生长和细胞迁移。最后，与MPC共培养的DRG似乎通过可溶性因子通讯增加了它们的神经营养功能。我们的研究结果表明，创伤肌肉来源的MPCs的神经营养功能是基本上等同于良好的特征人口的骨髓来源的MPCs，并建议的MPCs可以进一步发展为细胞疗法，以促进周围神经再生。
The success of peripheral nerve regeneration is governed by the rate and quality of axon bridging and myelination that occurs across the damaged region. Neurite growth and the migration of Schwann cells is regulated by neurotrophic factors produced as the nerve regenerates, and these processes can be enhanced by mesenchymal stem cells (MSCs), which also produce neurotrophic factors and other factors that improve functional tissue regeneration. Our laboratory has recently identified a population of mesenchymal progenitor cells (MPCs) that can be harvested from traumatized muscle tissue debrided and collected during orthopaedic reconstructive surgery. The objective of this study was to determine whether the traumatized muscle-derived MPCs exhibit neurotrophic function equivalent to that of bone marrow-derived MSCs. Similar gene- and protein-level expression of specific neurotrophic factors was observed for both cell types, and we localized neurogenic intracellular cell markers (brain-derived neurotrophic factor and nestin) to a subpopulation of both MPCs and MSCs. Furthermore, we demonstrated that the MPC-secreted factors were sufficient to enhance in vitro axon growth and cell migration in a chick embryonic dorsal root ganglia (DRG) model. Finally, DRGs in co-culture with the MPCs appeared to increase their neurotrophic function via soluble factor communication. Our findings suggest that the neurotrophic function of traumatized muscle-derived MPCs is substantially equivalent to that of the well-characterized population of bone marrow-derived MPCs, and suggest that the MPCs may be further developed as a cellular therapy to promote peripheral nerve regeneration.
登录
查看更多内容
DOI：
10.1083/jcb.150.5.1085
发表时间：
2000-09-04
期刊：
The Journal of cell biology
影响因子：
--
作者：
Lee JY;Qu-Petersen Z;Cao B;Kimura S;Jankowski R;Cummins J;Usas A;Gates C;Robbins P;Wernig A;Huard J
通讯作者：
Huard J
影响因子：
3.3
作者：
Jackson, W. M.;Aragon, A. B.;Djouad, F.;Song, Y.;Koehler, S. M.;Nesti, L. J.;Tuan, R. S.
通讯作者：
Tuan, R. S.
影响因子：
4.1
作者：
Gordon, Tessa
通讯作者：
Gordon, Tessa
影响因子：
--
作者：
Keilhoff, Gerburg;Goihl, Alexander;Fansa, Hisham
通讯作者：
Fansa, Hisham
影响因子：
3.7
作者：
Mahay, Daljeet;Terenghi, Giorgio;Shawcross, Susan G.
通讯作者：
Shawcross, Susan G.