Mitochondrial DNA analysis of field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) and of its relationship to H. zea.
Mitochondrial DNA analysis of field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) and of its relationship to H. zea.
复制标题
Helicoverpa Armigera（Lepidoptera：Noctuidae）及其与H. Zea的关系的线粒体DNA分析。
DOI：
10.1186/1471-2148-7-117
复制
发表时间：
2007-07-14
影响因子：
3.4
通讯作者：
Batterham P
中科院分区：
文献类型：
--
作者：
Behere GT;Tay WT;Russell DA;Heckel DG;Appleton BR;Kranthi KR;Batterham P
棉铃虫(Helicoverpa Armigera)和玉米象(H.Zea)分别是新旧大陆最重要的多食性鳞翅目害虫。棉铃虫和玉米象的分离很困难，通常只能通过生殖器的形态差异来实现。它们能够进行杂交，以产生可生育的后代。由于棉铃虫在东半球的广泛分布和植物寄主范围，棉铃虫的单一物种地位一直受到怀疑。本研究探讨了棉铃虫的全球遗传多样性及其与玉米的进化关系。我们获得了来自澳大利亚、布基纳法索、乌干达、中国、印度和巴基斯坦的249个棉铃虫个体的线粒体DNA(MtDNA)细胞色素氧化酶-I(COI)的部分(511bp)序列。部分COI基因内的单核苷酸多态(SNPs)将棉铃虫种群分为33个mtDNA单倍型。大洲之间共享单倍型，低F统计值和国家之间低核苷酸多样性(0.0017-0.0038)表明这种害虫的流动性很高。对四种主要害虫的系统发育分析表明，斑点夜蛾是烟粉虱的基生种，烟粉虱又是棉铃虫和玉米夜蛾的基生种。来自北美和南美的样本表明，在其分布范围内，H.Zea也是一个单一的物种。我们的数据揭示了棉铃虫和玉米之间很短的遗传距离，这种距离似乎是通过大约150万年前棉铃虫股票的创始人事件建立的。我们的线粒体DNA序列数据支持非洲、亚洲和澳大利亚棉铃虫的单一物种地位。这项研究中观察到的洲际基因流动的证据与已发表的该物种远距离迁徙能力的证据是一致的。在旧世界棉铃虫和新世界棉铃虫之间发现了高度的遗传相似性，这强调了在为任一种害虫制定害虫管理策略时，需要考虑这两种害虫的工作。
Helicoverpa armigera and H. zea are amongst the most significant polyphagous pest lepidopteran species in the Old and New Worlds respectively. Separation of H. armigera and H. zea is difficult and is usually only achieved through morphological differences in the genitalia. They are capable of interbreeding to produce fertile offspring. The single species status of H. armigera has been doubted, due to its wide distribution and plant host range across the Old World. This study explores the global genetic diversity of H. armigera and its evolutionary relationship to H zea. We obtained partial (511 bp) mitochondrial DNA (mtDNA) Cytochrome Oxidase-I (COI) sequences for 249 individuals of H. armigera sampled from Australia, Burkina Faso, Uganda, China, India and Pakistan which were associated with various host plants. Single nucleotide polymorphisms (SNPs) within the partial COI gene differentiated H. armigera populations into 33 mtDNA haplotypes. Shared haplotypes between continents, low F-statistic values and low nucleotide diversity between countries (0.0017 – 0.0038) suggests high mobility in this pest. Phylogenetic analysis of four major Helicoverpa pest species indicates that H. punctigera is basal to H. assulta, which is in turn basal to H. armigera and H. zea. Samples from North and South America suggest that H. zea is also a single species across its distribution. Our data reveal short genetic distances between H. armigera and H. zea which seem to have been established via a founder event from H. armigera stock at around 1.5 million years ago. Our mitochondrial DNA sequence data supports the single species status of H. armigera across Africa, Asia and Australia. The evidence for inter-continental gene flow observed in this study is consistent with published evidence of the capacity of this species to migrate over long distances. The finding of high genetic similarity between Old World H. armigera and New World H. zea emphasises the need to consider work on both pests when building pest management strategies for either.
登录
查看更多内容
DOI：
10.1098/rsbl.2003.0025
发表时间：
2003-08-07
影响因子：
4.7
作者：
Hebert, PDN;Ratnasingham, S;deWaard, JR
通讯作者：
deWaard, JR
影响因子：
4.9
作者：
Clement, M;Posada, D;Crandall, KA
通讯作者：
Crandall, KA
影响因子：
6.5
作者：
HUELSENBECK, JP;HILLIS, DM
通讯作者：
HILLIS, DM
影响因子：
3.9
作者：
HASEGAWA, M;KISHINO, H;YANO, TA
通讯作者：
YANO, TA
影响因子：
3.8
作者：
Brunner, PC;Chatzivassiliou, EK;Frey, JE
通讯作者：
Frey, JE