The effects of the covalent attachment of 3-(4-hydroxy-3,5-di-tert-butylphenyl) propyl amine to glutaraldehyde pretreated bovine pericardium on structural degeneration, oxidative modification, and calcification of rat subdermal implants.
The effects of the covalent attachment of 3-(4-hydroxy-3,5-di-tert-butylphenyl) propyl amine to glutaraldehyde pretreated bovine pericardium on structural degeneration, oxidative modification, and calcification of rat subdermal implants.
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DOI：
10.1002/jbm.a.35380
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发表时间：
2015-07
影响因子：
4.9
通讯作者：
Levy, Robert J.
中科院分区：
文献类型：
--
作者：
Christian, Abigail J.;Alferiev, Ivan S.;Connolly, Jeanne M.;Ischiropoulos, Harry;Levy, Robert J.
由戊二醛预处理的异种移植材料、猪主动脉瓣或牛心包（BP）制成的生物人工心脏瓣膜（BHV）广泛用于心脏手术。由于结构退化，BHV在临床应用中逐渐失效。以前我们报道过，在临床失败的BP-BHV外植体中存在二酪氨酸（一种氧化氨基酸）;未植入的BP中没有检测到二酪氨酸。在相同的研究中，BP在体外被证明对氧化损伤敏感，这可以通过用抗氧化剂3-（4-羟基-3，5-二叔丁基苯基）丙胺（DBP）共价修饰的BP来减轻。目前的研究比较了大鼠皮下植入戊二醛预处理的BP与用DBP或用于连接DBP的化学反应修饰的BP。所有BP外植体，无论DBP表现出减少羟脯氨酸和胶原酶的消化率增加。然而，与对照BP相比，DBP-BP外植体显示出对降低的外植体收缩温度（交联指数）的显著抑制。与BP相比，在BP-DBP和化学改性外植体中均观察到钙化显著缓解。在90天的外植体中未检测到酪氨酸。可以得出结论，大鼠皮下BP植入物经历钙化和非钙化结构变性，但没有形成二酪氨酸，不像临床BP外植体。
Bioprosthetic heart valves (BHV) fabricated from glutaraldehyde pretreated heterograft materials, porcine aortic valves or bovine pericardium (BP), are widely used in cardiac surgery. BHV progressively fail in clinical use due to structural degeneration. Previously we reported that dityrosine, an oxidized amino acid, was present in failed clinical BP-BHV explants; unimplanted BP had no detectable dityrosine. In the same studies BP were demonstrated in vitro to be susceptible to oxidative damage, that could be mitigated with BP covalently modified with the antioxidant, 3-(4-hydroxy-3,5-di-tert-butylphenyl)propyl amine (DBP). The present studies compared in rat subdermal implants glutaraldehyde pretreated BP to BP modified with either DBP or the chemical reactions used to link DBP. All BP explants regardless of DBP demonstrated reduced hydroxyproline and increased digestibility by collagenase. However, the DBP-BP explants showed significant inhibition of reduced explant shrink temperatures (an index of crosslinking) compared to control BP. Significant mitigation of calcification was observed in both the BP-DBP and chemically modified explants compared to BP. Dityrosine was not detectable in the 90 day explants. It is concluded that rat subdermal BP implants undergo both calcific and non-calcific structural degeneration, but without the formation of dityrosine, unlike clinical BP explants.
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影响因子：
3.9
作者：
WOESSNER, JF
通讯作者：
WOESSNER, JF
影响因子：
3.9
作者：
Au, V;Madison, SA
通讯作者：
Madison, SA
影响因子：
4.6
作者：
Connolly, Jeanne M.;Bakay, Marina A.;Alferiev, Ivan S.;Gorman, Robert C.;Gorman, Joseph H., III;Kruth, Howard S.;Ashworth, Paul E.;Kutty, Jaishankar K.;Schoen, Frederick J.;Bianco, Richard W.;Levy, Robert J.
通讯作者：
Levy, Robert J.
DOI：
10.1002/jbm.10293
发表时间：
2002-12-05
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
Sacks, MS;Schoen, FJ
通讯作者：
Schoen, FJ
影响因子：
14
作者：
Christian, Abigail J.;Lin, Hongqiao;Alferiev, Ivan S.;Connolly, Jeanne M.;Ferrari, Giovanni;Hazen, Stanley L.;Ischiropoulos, Harry;Levy, Robert J.
通讯作者：
Levy, Robert J.