Brassinosteroid-mediated stress tolerance in Arabidopsis shows interactions with abscisic acid, ethylene and salicylic acid pathways.
Brassinosteroid-mediated stress tolerance in Arabidopsis shows interactions with abscisic acid, ethylene and salicylic acid pathways.
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DOI：
10.1186/1471-2229-10-151
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发表时间：
2010-07-19
影响因子：
5.3
通讯作者：
Krishna P
中科院分区：
文献类型：
--
作者：
Divi UK;Rahman T;Krishna P
油菜素类固醇 (BR) 在植物发育中发挥着至关重要的作用，还可以促进对一系列非生物胁迫的耐受性。尽管人们对 BR 在植物发育中的作用了解很多，但 BR 控制植物应激反应和调节应激反应基因表达的机制尚不完全清楚。由于 BR 与其他植物激素相互作用，因此 BR 的胁迫耐受能力可能部分取决于其与其他胁迫激素的相互作用。利用一系列对脱落酸 (ABA)、乙烯 (ET)、茉莉酸 (JA) 和水杨酸 (SA) 缺乏或不敏感的拟南芥突变体，我们研究了 24-表油菜素 (EBR) 对这些突变体基本耐热性和耐盐性的影响。除 SA 不敏感的 npr1-1 突变体外，EBR 对耐热性的积极影响（与野生型成比例）在所有研究的突变体中都很明显。 EBR 可以将 ET 不敏感的 ein2 突变体从对盐胁迫诱导的种子萌发抑制的超敏反应中拯救出来，但在提高 eto1-1（ET 过度生产者）和 npr1-1 幼苗对盐的存活率方面仍然无效。与野生型相比，EBR 在 ABA 缺陷型 aba1-1 突变体中的积极作用明显更大，表明 ABA 掩盖了植物胁迫反应中的 BR 效应。 EBR处理增加了野生型和突变型幼苗中各种激素标记基因的表达，尽管水平不同。这些结果共同表明，氧化还原敏感蛋白 NPR1 (NONEXPRESSOR OF PATHOGENESIS-RELATED GENES1) 是 SA 介导的防御基因的主要调节因子，可能是 EBR 介导的耐热性和耐盐性增加的关键组成部分，但它不是 EBR 介导的 PR-1 (PATHOGENESIS-RELATED1) 基因表达诱导所必需的； BR 可以独立发挥抗压力作用，也可以通过与其他激素的相互作用发挥抗压力作用； ABA 在应激期间抑制 BR 效应； BR 与其他激素共享转录目标。
Brassinosteroids (BRs) play crucial roles in plant development and also promote tolerance to a range of abiotic stresses. Although much has been learned about their roles in plant development, the mechanisms by which BRs control plant stress responses and regulate stress-responsive gene expression are not fully known. Since BR interacts with other plant hormones, it is likely that the stress tolerance conferring ability of BR lies in part in its interactions with other stress hormones. Using a collection of Arabidopsis mutants that are either deficient in or insensitive to abscisic acid (ABA), ethylene (ET), jasmonic acid (JA) and salicylic acid (SA), we studied the effects of 24-epibrassinloide (EBR) on basic thermotolerance and salt tolerance of these mutants. The positive impact of EBR on thermotolerance in proportion to wild type was evident in all mutants studied, with the exception of the SA-insensitive npr1-1 mutant. EBR could rescue the ET-insensitive ein2 mutant from its hypersensitivity to salt stress-induced inhibition of seed germination, but remained ineffective in increasing the survival of eto1-1 (ET-overproducer) and npr1-1 seedlings on salt. The positive effect of EBR was significantly greater in the ABA-deficient aba1-1 mutant as compared to wild type, indicating that ABA masks BR effects in plant stress responses. Treatment with EBR increased expression of various hormone marker genes in both wild type and mutant seedlings, although to different levels. These results together indicate that the redox-sensitive protein NPR1 (NONEXPRESSOR OF PATHOGENESIS-RELATED GENES1), a master regulator of SA-mediated defense genes, is likely a critical component of EBR-mediated increase in thermotolerance and salt tolerance, but it is not required for EBR-mediated induction of PR-1 (PATHOGENESIS-RELATED1) gene expression; that BR exerts anti-stress effects independently as well as through interactions with other hormones; that ABA inhibits BR effects during stress; and that BR shares transcriptional targets with other hormones.
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影响因子：
56.9
作者：
He, JX;Gendron, JM;Wang, ZY
通讯作者：
Wang, ZY
影响因子：
11.6
作者：
CAO, H;BOWLING, SA;DONG, XN
通讯作者：
DONG, XN
影响因子：
7.2
作者：
He, ZH;He, DZ;Kohorn, BD
通讯作者：
Kohorn, BD
DOI：
10.1073/pnas.92.14.6602
发表时间：
1995-07-03
影响因子：
11.1
作者：
DELANEY, TP;FRIEDRICH, L;RYALS, JA
通讯作者：
RYALS, JA
影响因子：
11.6
作者：
Bowling, SA;Clarke, JD;Dong, XN
通讯作者：
Dong, XN