Characterization of Calcium Phosphate Spherical Particles in the Subretinal Pigment Epithelium-Basal Lamina Space in Aged Human Eyes.
Characterization of Calcium Phosphate Spherical Particles in the Subretinal Pigment Epithelium-Basal Lamina Space in Aged Human Eyes.
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DOI：
10.1016/j.xops.2021.100053
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发表时间：
2021-09
影响因子：
--
通讯作者：
Lengyel, Imre
中科院分区：
文献类型：
--
作者：
Pilgrim, Matthew G.;Marouf, Salma;Fearn, Sarah;Csincsik, Lajos;Kortvely, Elod;Knowles, Jonathan C.;Malek, Goldis;Thompson, Richard B.;Lengyel, Imre
在视网膜色素上皮(RPE)基底层(BL)和Bruchs膜内胶原层之间的间隙(亚RPE-BL间隙)的细胞外沉积中发现了由羟基磷灰石或白锁石形成的微米级球体。本研究旨在研究老年性黄斑变性(AMD)患者的老年眼的形态特征、结构和分布。实验研究。从伦敦大学学院眼科研究所和穆尔菲尔德眼科医院组织库或Advance Sight Network获得了5只具有不同程度RPE-BL沉积的人眼。据报道，两只眼有AMD的临床指征(年龄76-87岁)，而3只眼被认为是健康的(年龄69-91岁)。用石蜡包埋RPE-BL下沉积的身体眼，切片厚度4~10μm，用高分辨扫描电子显微镜、能量色散X射线能谱和飞行时间二次离子质谱仪对小球进行鉴定和表征。球体的高分辨率扫描电子显微镜照片，球体的尺寸-频率分布，包括平均直径，以及粒子在中周轴上的分布。还得到了元素图和飞行时间二次离子质谱图。在老年眼的中心轴、中周轴和远周轴上，小球的沉淀普遍存在。沿该轴方向球体的出现频率无明显差异。然而，统计分析表明，这些区域的球体大小有显著差异。深入分析发现，老年性黄斑变性患者视网膜色素上皮下间隙球体直径(中位数为1.64μm)明显大于健康老年组(中位数直径为1.16μm)。最后，球体在表面形貌和内部结构上表现出很大的变化。在老年人眼的中央-外周轴线上，亚RPE-BL空间中的小球沉淀是普遍存在的。然而，有AMD临床症状的眼球的大小和频率与无AMD的眼相比存在显著差异，提示小球的大小和频率可能与AMD有关。
Micrometer-sized spherules formed of hydroxyapatite or whitlockite were identified within extracellular deposits that accumulate in the space between the basal lamina (BL) of retinal pigment epithelium (RPE) and the inner collagenous layer of Bruch’s membrane (sub-RPE–BL space). This investigation aimed to characterize the morphologic features, structure, and distribution of these spherules in aged human eyes with and without clinical indications of age-related macular degeneration (AMD). Experimental study. Five human eyes with varying degrees of sub-RPE–BL deposits were obtained from the University College London Institute of Ophthalmology and Moorfield’s Eye Hospital Tissue Repository or the Advancing Sight Network. Two eyes were reported as having clinical indications of AMD (age, 76–87 years), whereas 3 were considered healthy (age, 69–91 years). Cadaveric eyes with sub-RPE–BL deposits were embedded in paraffin wax and sectioned to a thickness of 4-10 μm. Spherules were identified and characterized using high-resolution scanning electron microscopy (SEM), energy-dispersive x-ray spectroscopy, and time-of-flight secondary ion mass spectroscopy. High-resolution scanning electron micrographs of spherules, the size-frequency distribution of spherules including average diameter, and the distribution of particles across the central-peripheral axis. Elemental maps and time-of-flight secondary ion mass spectra also were obtained. The precipitation of spherules is ubiquitous across the central, mid-peripheral, and far-peripheral axis in aged human eyes. No significant difference was found in the frequency of spherules along this axis. However, statistical analysis indicated that spherules exhibited significantly different sizes in these regions. In-depth analysis revealed that spherules in the sub-RPE–BL space of eyes with clinical signs of AMD were significantly larger (median diameter, 1.64 μm) than those in healthy aged eyes (median diameter, 1.16 μm). Finally, spherules showed great variation in surface topography and internal structure. The precipitation of spherules in the sub-RPE–BL space is ubiquitous across the central–peripheral axis in aged human eyes. However, a marked difference exists in the size and frequency of spherules in eyes with clinical signs of AMD compared to those without, suggesting that the size and frequency of spherules may be associated with AMD.
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影响因子：
13.7
作者：
Domalpally, Amitha;Clemons, Traci E.;Chew, Emily Y.
通讯作者：
Chew, Emily Y.
影响因子：
2.1
作者：
Ukalovic, Kresimir;Cao, Sijia;Matsubara, Joanne A.
通讯作者：
Matsubara, Joanne A.
影响因子：
41.2
作者：
Bertazzo S;Gentleman E;Cloyd KL;Chester AH;Yacoub MH;Stevens MM
通讯作者：
Stevens MM
影响因子：
2.1
作者：
Csincsik, Lajos;MacGillivray, Thomas J.;Lengyel, Imre
通讯作者：
Lengyel, Imre
DOI：
10.1111/j.1442-9071.1980.tb01670.x
发表时间：
1980-01-01
期刊：
AUSTRALIAN JOURNAL OF OPHTHALMOLOGY
影响因子：
--
作者：
SARKS, SH
通讯作者：
SARKS, SH