Multi-platform 'Omics Analysis of Human Ebola Virus Disease Pathogenesis.
Multi-platform 'Omics Analysis of Human Ebola Virus Disease Pathogenesis.
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DOI：
10.1016/j.chom.2017.10.011
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发表时间：
2017-12-13
影响因子：
30.3
通讯作者：
Kawaoka Y
中科院分区：
文献类型：
--
作者：
Eisfeld AJ;Halfmann PJ;Wendler JP;Kyle JE;Burnum-Johnson KE;Peralta Z;Maemura T;Walters KB;Watanabe T;Fukuyama S;Yamashita M;Jacobs JM;Kim YM;Casey CP;Stratton KG;Webb-Robertson BM;Gritsenko MA;Monroe ME;Weitz KK;Shukla AK;Tian M;Neumann G;Reed JL;van Bakel H;Metz TO;Smith RD;Waters KM;N'jai A;Sahr F;Kawaoka Y
人类埃博拉病毒病（EVD）的发病机制很复杂。埃博拉病毒病的特点是病毒高度复制和传播、免疫反应失调、病毒和宿主介导的广泛组织损伤以及凝血障碍。为了阐明宿主反应如何影响埃博拉病毒病病理生理学，我们对埃博拉病毒病患者的外周血单核细胞和血浆进行了多平台组学分析。我们的研究结果表明，EVD 分子特征与脓毒症的分子特征重叠，暗示胰腺酶导致致命性 EVD 的组织损伤，并表明埃博拉病毒感染可能诱导异常中性粒细胞，其活性可以解释致命性 EVD 的特征。此外，综合生物标志物预测从不同的数据平台中识别出假定的生物标志物，这些生物标志物在感染后早期就区分了幸存者和死亡者。这项工作揭示了对埃博拉病毒病发病机制的深入了解，提出了生物标志物识别的有效方法，并为进一步分析人类埃博拉病毒病严重程度提供了重要的社区资源。艾斯菲尔德等人。综合评估了埃博拉病毒病（EVD）患者血浆和外周免疫细胞中宿主分子的变化。他们的结果提出了埃博拉病毒病发病机制的新机制和用于预测埃博拉病毒病结果的推定生物标志物。此外，与这项工作相关的数据集是进一步研究的重要社区资源。
The pathogenesis of Human Ebola virus disease (EVD) is complex. EVD is characterized by high levels of virus replication and dissemination, dysregulated immune responses, extensive virus- and host-mediated tissue damage, and disordered coagulation. To clarify how host responses contribute to EVD pathophysiology, we performed multi-platform ‘omics analysis of peripheral blood mononuclear cells and plasma from EVD patients. Our results indicate that EVD molecular signatures overlap with those of sepsis, imply that pancreatic enzymes contribute to tissue damage in fatal EVD, and suggest that EBOV infection may induce aberrant neutrophils whose activity could explain hallmarks of fatal EVD. Moreover, integrated biomarker prediction identified putative biomarkers from different data platforms that differentiated survivors and fatalities early after infection. This work reveals insight into EVD pathogenesis, suggests an effective approach for biomarker identification, and provides an important community resource for further analysis of human EVD severity. Eisfeld et al. comprehensively evaluated changes in host molecules in plasma and peripheral immune cells of Ebola virus disease (EVD) patients. Their results suggest new mechanisms of EVD pathogenesis and putative biomarkers for predicting EVD outcomes. Moreover, datasets associated with this work are an important community resource for further research.
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DOI：
10.1186/cc14003
发表时间：
2014-08-01
期刊：
Critical care (London, England)
影响因子：
--
作者：
Darcy CJ;Minigo G;Piera KA;Davis JS;McNeil YR;Chen Y;Volkheimer AD;Weinberg JB;Anstey NM;Woodberry T
通讯作者：
Woodberry T
影响因子：
4.4
作者：
Martinez, Fernando O.;Gordon, Siamon;Mantovani, Alberto
通讯作者：
Mantovani, Alberto
影响因子：
158.5
作者：
Schieffelin, J. S.;Shaffer, J. G.;Garry, R. F.
通讯作者：
Garry, R. F.
影响因子：
7.5
作者：
Lacroix, Romaric;Dignat-George, Francoise
通讯作者：
Dignat-George, Francoise
影响因子：
3.4
作者：
Maiolica, A;Borsotti, D;Rappsilber, J
通讯作者：
Rappsilber, J