High expression of MAPK-14 promoting the death of chondrocytes is an important signal of osteoarthritis process.
High expression of MAPK-14 promoting the death of chondrocytes is an important signal of osteoarthritis process.
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作者：
Deng Z;Hu X;Alahdal M;Liu J;Zhao Z;Chen X;Xie J;Duan L;Wang D;Li W
骨关节炎（OA）是世界范围内最常见的退行性疾病之一。许多研究者正在研究OA的发病机制，但目前尚不清楚。OA相关枢纽基因的筛选和验证是探索其潜在分子机制的重要组成部分。因此，本研究旨在通过生物信息学、qPCR、荧光和碘化丙啶染色等方法探讨和验证枢纽基因在OA中的作用机制。基因芯片数据集，收集于基因表达数据库（Gene Expression Omnibus，GEO），共45个样本，其中骨关节炎膝关节样本23个，正常膝关节样本22个。共鉴定出265个差异表达基因，其中上调基因199个，下调基因66个。中枢基因MAPK-14、PTPRC、PTPN 12表达上调，而B 9D 1表达下调。为了进一步证实筛选的差异基因在人软骨细胞中的表达，从关节置换手术中提取人软骨细胞，并用甲苯胺蓝染色进行鉴定。与正常软骨细胞相比，OA软骨细胞COL I蛋白表达量高，COL II蛋白表达量低。MAPK-14、PTPRC和PTPN 12在OA软骨细胞中的表达水平显著高于B 9D 1在正常软骨细胞中的表达水平。碘化丙啶染色显示OA软骨细胞的炎性坏死较正常软骨细胞明显增加。MAPK-14的高表达作为软骨细胞死亡的促进剂和骨关节炎过程的重要信号起作用。
Osteoarthritis (OA) is one of the most common degenerative diseases worldwide. Many researchers are studying the pathogenesis of OA, however, it is still unclear. Screening and validation of OA relevant hub genes are an important part of exploring their potential molecular mechanism. Therefore, this study aims to explore and verify the mechanisms of hub genes in the OA by bioinformatics, qPCR, fluorescence and propidium iodide staining. Microarray datasets , and were collected in the Gene Expression Omnibus (GEO), including 45 samples, which divided into 23 osteoarthritis knee joint samples and 22 samples of normal knee joint. Thereafter, 265 differentiallyexpressedgenes (DEGs) were identified in all, which divided into 199 upregulated genes and 66 downregulated genes. The hub genes MAPK-14, PTPRC, PTPN12 were upregulated, while B9D1 was downregulated. In order to further confirm the expression of screening differential genes in human chondrocytes, the human chondrocytes were extracted from a joint replacement surgery and stained with toluidine blue for identification. Compared with normal chondrocytes, OA chondrocytes had high expression of COL I protein and low expression of COL II protein. The expression levels of MAPK-14, PTPRC and PTPN12 in OA chondrocytes were significantly higher than the expression levels of B9D1 in normal chondrocytes. Moreover, the inflammatory necrosis of OA chondrocytes was increased compared with the normal chondrocytes by propidium iodide staining. The high expression of MAPK-14 works as a promoter of chondrocytes death and an important signal of the osteoarthritis process.
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影响因子：
3.3
作者：
Rahman W;Patel R;Dickenson AH
通讯作者：
Dickenson AH
DOI：
10.1038/nri3477
发表时间：
2013-08
期刊：
Nature reviews. Immunology
影响因子：
--
作者：
通讯作者：
--
影响因子：
7
作者：
Torres, L.;Dunlop, D. D.;Sharma, L.
通讯作者：
Sharma, L.
影响因子：
2.9
作者：
StrangeVognsen, HH;Arnbjerg, J;Hannibal, J
通讯作者：
Hannibal, J
影响因子：
5.5
作者：
Croft, P
通讯作者：
Croft, P