Single channel properties of pannexin-1 and connexin-43 hemichannels and P2X7 receptors in astrocytes cultured from rodent spinal cords.
Single channel properties of pannexin-1 and connexin-43 hemichannels and P2X7 receptors in astrocytes cultured from rodent spinal cords.
复制标题
DOI：
10.1002/glia.24250
复制
发表时间：
2022-12
期刊：
影响因子：
6.2
通讯作者：
Bennett, Michael V. L.
中科院分区：
文献类型：
--
作者：
Garre, Juan Mauricio;Bukauskas, Feliksas F.;Bennett, Michael V. L.
星形胶质细胞表达参与嘌呤能信号传导的表面通道。在这些通道中，泛连接蛋白-1（Px 1）和连接蛋白-43（Cx43）半通道（HC）释放ATP，其直接或通过其衍生物经由嘌呤能受体作用于神经元和神经胶质。虽然HC是功能性的，即，在生理和病理条件下开放和关闭，星形胶质细胞中Px 1 HC的单通道特性尚未确定。在这里，我们在表达人Px 1-YFP的HeLa细胞中开发了双电压钳技术，然后将该系统应用于啮齿动物脊髓星形胶质细胞，以比较其单通道特性与其他表面通道，即，Cx43 HC和P2 X7受体（P2 X7 Rs）。在细胞附着的贴片中记录通道，并在全细胞电压钳中通过另一个移液管施加斜坡循环来诱发通道。在HeLa Px 1-YFP细胞和脊髓星形胶质细胞中，Px 1 HC的平均单位电导相当，分别为~42 pS和~48 pS。基于其单一的电导，电压依赖性，和单一的活动后，药理学和基因沉默，在星形胶质细胞中的Px 1 HC可以区分Cx43 HC和P2 X7 Rs。在斜坡期间，在对照星形胶质细胞中，Px 1 HC和P2 X7 Rs的通道活性大于Cx43 HC。成纤维细胞生长因子1（FGF-1）处理后，星形胶质细胞Px 1 HCs的单位活性降低，Cx43 HCs和P2 X7 Rs的单位活性增加。总之，我们解决了三个不同的表面通道参与嘌呤信号在脊髓星形胶质细胞，这是差异调制的FGF-1，参与神经发育，炎症和修复的生长因子的单通道特性。
Astrocytes express surface channels involved in purinergic signaling. Among these channels, pannexin-1 (Px1) and connexin-43 (Cx43) hemichannels (HCs) release ATP that acts directly, or through its derivatives, on neurons and glia via purinergic receptors. Although HCs are functional, i.e., open and close under physiological and pathological conditions, single channel properties of Px1 HCs in astrocytes has not been defined. Here, we developed a dual voltage clamp technique in HeLa cells expressing human Px1-YFP, and then applied this system to rodent spinal astrocytes to compare their single channel properties with other surface channels, i.e., Cx43 HCs and P2X7 receptors (P2X7Rs). Channels were recorded in cell attached patches and evoked with ramp cycles applied through another pipette in whole cell voltage clamp. The mean unitary conductances of Px1 HCs were comparable in HeLa Px1-YFP cells and spinal astrocytes, ~42 pS and ~48 pS, respectively. Based on their unitary conductance, voltage-dependence, and unitary activity after pharmacological and gene silencing, Px1 HCs in astrocytes could be distinguished from Cx43 HCs and P2X7Rs. Channel activity of Px1 HCs and P2X7Rs was greater than that of Cx43 HCs in control astrocytes during ramps. Unitary activity of Px1 HCs was decreased and that of Cx43 HCs and P2X7Rs increased in astrocytes treated with fibroblast growth factor 1 (FGF-1). In summary, we resolved single channel properties of three different surface channels involved in purinergic signaling in spinal astrocytes, which were differentially modulated by FGF-1, a growth factor involved in neurodevelopment, inflammation and repair.
登录
查看更多内容
影响因子：
5.6
作者：
Battulin N;Kovalzon VM;Korablev A;Serova I;Kiryukhina OO;Pechkova MG;Bogotskoy KA;Tarasova OS;Panchin Y
通讯作者：
Panchin Y
影响因子：
5.1
作者：
Klimaschewski L;Claus P
通讯作者：
Claus P
DOI：
10.1073/pnas.1320401111
发表时间：
2014-07-22
影响因子：
11.1
作者：
Kang, Wenfei;Balordi, Francesca;Hebert, Jean M.
通讯作者：
Hebert, Jean M.
DOI：
10.1073/pnas.2233464100
发表时间：
2003-11-11
影响因子：
11.1
作者：
Bruzzone, R;Hormuzdi, SG;Monyer, H
通讯作者：
Monyer, H
DOI：
10.1073/pnas.012589799
发表时间：
2002-01-08
影响因子：
11.1
作者：
Contreras, JE;Sánchez, HA;Sáez, JC
通讯作者：
Sáez, JC