Metabolomics-guided analysis reveals a two-step epimerization of deoxynivalenol catalyzed by the bacterial consortium IFSN-C1
Metabolomics-guided analysis reveals a two-step epimerization of deoxynivalenol catalyzed by the bacterial consortium IFSN-C1
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代谢组学引导的分析揭示了细菌群 IFSN-C1 催化的脱氧雪腐镰刀菌烯醇的两步差向异构化
DOI：
10.1007/s00253-020-10673-1
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发表时间：
2020-05
影响因子：
5
通讯作者：
Xu Jianhong
中科院分区：
文献类型：
--
作者：
Wang Gang;Wang Yanxia;Man Huizi;Lee Yin-Won;Shi Jianrong;Xu Jianhong
脱氧雪腐镰刀菌烯醇(DON)普遍存在于小麦及其衍生食品中，对人类健康构成威胁。生物降解是一种高效、环保的霉菌毒素脱毒方法。因此，了解DON的生物降解机理具有重要意义。在此，我们报道了应用代谢组学方法分析从江苏省小麦叶片中分离的细菌对DON的降解。代谢组学分析结合核磁共振分析揭示了主要降解产物3-酮-酮和次要降解产物3-表-酮。进一步的研究表明，DON通过中间的3-酮-酮经历了两步的异构化。对微生物群落16S rRNA元基因组的序列分析表明，在细菌联盟与DON共培养过程中，Achromobacter、Sphingopyxis和Sphingomonas三个细菌属的丰度显著增加。进一步调查发现，德沃西亚属(Devosia sp.)可能对3-酮-酮的异构化负责。这些发现揭示了DON在生物降解过程中的分解代谢途径，并说明了在生物降解研究中使用代谢组学方法的潜力。
Deoxynivalenol (DON) is commonly found in wheat and wheat-derived foods, posing a threat to human health. Biodegradation is an efficient and eco-friendly measure for mycotoxin detoxification. Understanding the mechanism of DON biodegradation is hence of great importance. Herein, we report the application of metabolomics methods for the analysis of DON degradation by a bacterial consortium isolated from wheat leaves collected in Jiangsu Province. Metabolomics analysis combined with a nuclear magnetic resonance analysis revealed the main degradation product, 3-keto-DON, and a minor degradation product, 3-epi-DON. Further study illustrated that DON underwent a two-step epimerization through the intermediate 3-keto-DON. Sequencing analysis of the 16S rRNA metagenome of the microorganismal community suggested that the abundance of three bacterial genera, Achromobacter, Sphingopyxis, and Sphingomonas, substantially increased during the coculture of bacterial consortium and DON. Further investigation revealed that Devosia sp. might be responsible for the epimerization of 3-keto-DON. These findings shed light on the catabolic pathways of DON during biodegradation and illustrate the potential of using metabolomics approaches in biodegradation studies.
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