Oral administration of inactivated porcine epidemic diarrhea virus activate DCs in porcine Peyer's patches.
Oral administration of inactivated porcine epidemic diarrhea virus activate DCs in porcine Peyer's patches.
复制标题
口服灭活的猪流行性腹泻病毒可激活猪派尔氏淋巴集结中的 DC。
DOI：
10.1186/s12917-018-1568-z
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发表时间：
2018-08-16
影响因子：
2.6
通讯作者：
Yang Q
中科院分区：
文献类型：
--
作者：
Yuan C;Zhang E;Huang L;Wang J;Yang Q
Peyer’s patches (PPs) can be considered as the immune site of the intestine. Within PPs, Dendritic cells (DCs) can uptake antigens from the gut lumen by extending dendrites into epithelium, and process it and then present to lymphocytes, which effectively antigen produces an immune response. Porcine epidemic diarrhea virus (PEDV) is the causative agent of porcine epidemic diarrhea (PED), an acute and highly contagious enteric viral disease. The interaction between inactivated porcine epidemic diarrhea virus and porcine monocyte-derived dendritic cells (Mo-DCs) has been reported. However, little is known about the interaction between inactivated PEDV and DCs in porcine PPs. In this study, for the first time we investigated the role of DCs in porcine PPs after oral administration inactivated PEDV. Firstly, a method to isolate DCs from porcine PPs was established, in which the purity of SWC3a+/MHC-II+ DCs was more than 90%. Our findings clearly indicate that DCs in porcine PPs after oral administration of inactivated PEDV not only stimulated the proliferation of allogeneic lymphocytes, but also secreted cytokines (IL-1, IL-4). Furthermore, the number of DCs and IgA+ cells in porcine intestinal mucosal significantly increased and the levels of anti-PEDV specific IgG antibody in the serum and SIgA antibody in the feces increased after oral administration inactivated PEDV. Our findings indicate that oral administration of inactivated PEDV activate DCs in porcine Peyer’s patches and inactivated PEDV may be a useful and safe vaccine to trigger adaptive immunity.
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影响因子：
2
作者：
Jung C;Hugot JP;Barreau F
通讯作者：
Barreau F
DOI：
10.1084/jem.190.2.229
发表时间：
1999-07-19
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Iwasaki A;Kelsall BL
通讯作者：
Kelsall BL
影响因子：
4.3
作者：
Monger, V. R.;Stegeman, J. A.;Loeffen, W. L. A.
通讯作者：
Loeffen, W. L. A.
影响因子：
4.9
作者：
Van Kruiningen, HJ;West, AB;Holmes, KA
通讯作者：
Holmes, KA
影响因子：
7.8
作者：
Owen, Jennifer L.;Sahay, Bikash;Mohamadzadeh, Mansour
通讯作者：
Mohamadzadeh, Mansour