Infectivity of severe acute respiratory syndrome coronavirus 2 in children compared with adults.
Infectivity of severe acute respiratory syndrome coronavirus 2 in children compared with adults.
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DOI：
10.1503/cmaj.210263
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发表时间：
2021-04-26
期刊：
影响因子：
--
通讯作者：
Poliquin G
中科院分区：
文献类型：
--
作者：
Bullard J;Funk D;Dust K;Garnett L;Tran K;Bello A;Strong JE;Lee SJ;Waruk J;Hedley A;Alexander D;Van Caeseele P;Loeppky C;Poliquin G
儿童在严重急性呼吸综合征冠状病毒2型（SARS-CoV-2）传播和社区传播中的作用尚不清楚。我们的目的是量化SARS-CoV-2在儿童鼻咽样本中的感染性，并与成人进行比较。我们从2020年3月至12月期间在马尼托巴的成人和儿童2019冠状病毒病（COVID-19）病例及其SARS-CoV-2检测呈阳性的接触者中获得了鼻咽拭子。我们比较了成人和儿童的细胞培养中的病毒生长、来自SARS-CoV-2包膜（E）基因的逆转录聚合酶链反应（RT-PCR）的循环阈值以及50%组织培养感染剂量（TCID 50/mL）。在305份RT-PCR检测SARS-CoV-2阳性样本中，97份来自10岁或以下儿童，78份来自11-17岁儿童，130份来自成人（≥ 18岁）。31%的样本中存在培养物中的病毒生长，包括18份（19%）来自10岁或10岁以下儿童的样本，18份（23%）来自11-17岁儿童的样本和57份（44%）来自成人的样本（儿童与成人，比值比0.45，95%置信区间[CI] 0.28-0.72）。10岁或以下儿童的周期阈值为25.1（95% CI 17.7-31.3），11-17岁儿童为22.2（95% CI 18.3-29.0），成人为18.7（95% CI 17.9-30.4）（p < 0.001）。11-17岁儿童的中位TCID 50/mL（316，四分位距[IQR] 178-2125）显著低于成人（5620，IQR 1171 - 17 800，p < 0.001）。循环阈值是儿童和成人阳性培养的准确预测因子（受试者-操作者曲线下面积，0.87，95% CI 0.81-0.93 v. 0.89，95% CI 0.83-0.96，p = 0.6）。与成人相比，鼻咽拭子检测SARS-CoV-2阳性的儿童在培养中生长病毒的可能性较小，并且具有较高的循环阈值和较低的病毒浓度，这表明儿童不是SARS-CoV-2传播的主要驱动因素。
The role of children in the transmission and community spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is unclear. We aimed to quantify the infectivity of SARS-CoV-2 in nasopharyngeal samples from children compared with adults. We obtained nasopharyngeal swabs from adult and pediatric cases of coronavirus disease 2019 (COVID-19) and from their contacts who tested positive for SARS-CoV-2 in Manitoba between March and December 2020. We compared viral growth in cell culture, cycle threshold values from the reverse transcription polymerase chain reaction (RT-PCR) of the SARS-CoV-2 envelope (E) gene and the 50% tissue culture infective dose (TCID50/mL) between adults and children. Among 305 samples positive for SARS-CoV-2 by RT-PCR, 97 samples were from children aged 10 years or younger, 78 were from children aged 11–17 years and 130 were from adults (≥ 18 yr). Viral growth in culture was present in 31% of samples, including 18 (19%) samples from children 10 years or younger, 18 (23%) from children aged 11–17 years and 57 (44%) from adults (children v. adults, odds ratio 0.45, 95% confidence interval [CI] 0.28–0.72). The cycle threshold was 25.1 (95% CI 17.7–31.3) in children 10 years or younger, 22.2 (95% CI 18.3–29.0) in children aged 11–17 years and 18.7 (95% CI 17.9–30.4) in adults (p < 0.001). The median TCID50/mL was significantly lower in children aged 11–17 years (316, interquartile range [IQR] 178–2125) than adults (5620, IQR 1171 to 17 800, p < 0.001). Cycle threshold was an accurate predictor of positive culture in both children and adults (area under the receiver-operator curve, 0.87, 95% CI 0.81–0.93 v. 0.89, 95% CI 0.83–0.96, p = 0.6). Compared with adults, children with nasopharyngeal swabs that tested positive for SARS-CoV-2 were less likely to grow virus in culture, and had higher cycle thresholds and lower viral concentrations, suggesting that children are not the main drivers of SARS-CoV-2 transmission.
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影响因子：
158.5
作者：
Reichert, TA;Sugaya, N;Tashiro, M
通讯作者：
Tashiro, M
影响因子：
82.9
作者：
Adam, Dillon C.;Wu, Peng;Cowling, Benjamin J.
通讯作者：
Cowling, Benjamin J.
DOI：
10.1073/pnas.2011802117
发表时间：
2020-09-08
影响因子：
11.1
作者：
Lau, Max S. Y.;Grenfell, Bryan;Lopman, Ben
通讯作者：
Lopman, Ben
影响因子：
11.8
作者：
Guilamo-Ramos, Vincent;Benzekri, Adam;Perlman, David C.
通讯作者：
Perlman, David C.
影响因子：
19
作者：
Heavey, Laura;Casey, Geraldine;McDarby, Geraldine
通讯作者：
McDarby, Geraldine