Expression of beta 1, beta 3, beta 4, and beta 5 integrins by human epidermal keratinocytes and non-differentiating keratinocytes
Expression of beta 1, beta 3, beta 4, and beta 5 integrins by human epidermal keratinocytes and non-differentiating keratinocytes
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人表皮角质形成细胞和非分化角质形成细胞表达β1、β3、β4和β5整合素
DOI：
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发表时间：
1991
影响因子：
7.8
通讯作者：
Fiona M. Watt
中科院分区：
文献类型：
--
作者：
Josephine C. Adams;Fiona M. Watt
我们比较了培养的人表皮角质形成细胞和一株未分化角质形成细胞(NDK)的粘附性和整合素表达谱。两种类型的细胞都能附着于纤维连接蛋白、层粘连蛋白以及I型和IV型胶原，但NDK黏附更快，并且在较低的蛋白质包被浓度下黏附。抗体阻断实验表明，两种细胞对纤维连接蛋白的黏附均由α5β1整合素介导，层粘连蛋白由α3β1与α2β1协同介导。角质形成细胞通过α2β1与胶原黏附，但抗α2抗体不能抑制NDK与胶原的黏附。这两种类型的细胞都通过含有αv整合素的整合素粘附于Vitronectin。免疫沉淀结果显示，除α2β1、α3β1和α5β1外，角质形成细胞和NDK均表达α6β4和αvβ5。NDK比正常角质形成细胞表达更高水平的整合素。NDK，但不是正常角质形成细胞，表达αvβ1和αvβ3；它们也表达α1β1，这是一种在正常角质形成细胞上不一致检测到的整合素。免疫荧光实验显示，在正常角质形成细胞的分层培养中，整合素的表达仅局限于基底层的细胞；终末分化细胞未见染色。相反，NDK群体中的所有细胞都是整合素阳性。我们的观察表明，NDK的粘附性不同于正常角质形成细胞，反映了整合素在细胞表面表达的类型、表达水平和分布的不同。因此，NDK具有许多区别于正常基底角质形成细胞的特征。
We have compared the adhesive properties and integrin expression profiles of cultured human epidermal keratinocytes and a strain of nondifferentiating keratinocytes (ndk). Both cell types adhered to fibronectin, laminin, and collagen types I and IV, but ndk adhered more rapidly and at lower coating concentrations of the proteins. Antibody blocking experiments showed that adhesion of both cell types to fibronectin was mediated by the alpha 5 beta 1 integrin and to laminin by alpha 3 beta 1 in synergy with alpha 2 beta 1. Keratinocytes adhered to collagen with alpha 2 beta 1, but an antibody to alpha 2 did not inhibit adhesion of ndk to collagen. Both cell types adhered to vitronectin by alpha v-containing integrins. Immunoprecipitation of surface-iodinated and metabolically labeled cells showed that in addition to alpha 2 beta 1, alpha 3 beta 1, and alpha 5 beta 1, both keratinocytes and ndk expressed alpha 6 beta 4 and alpha v beta 5. ndk expressed all these integrins at higher levels than normal keratinocytes. ndk, but not normal keratinocytes, expressed alpha v beta 1 and alpha v beta 3; they also expressed alpha 1 beta 1, an integrin that was not consistently detected on normal keratinocytes. Immunofluorescence experiments showed that in stratified cultures of normal keratinocytes integrin expression was confined to cells in the basal layer; terminally differentiating cells were unstained. In contrast, all cells in the ndk population were integrin positive. Our observations showed that the adhesive properties of ndk differ from normal keratinocytes and reflect differences in the type of integrins expressed, the level of expression and the distribution of integrins on the cell surface. ndk thus have a number of characteristics that distinguish them from normal basal keratinocytes.
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影响因子：
20.3
作者：
S. Greenberg;Rosenthal Ds;T. A. Greeley;R. Tantravahi;R. Handin
通讯作者：
S. Greenberg;Rosenthal Ds;T. A. Greeley;R. Tantravahi;R. Handin
通过单克隆抗体在人肺肿瘤上鉴定出膜相关糖蛋白 (gp 160)。
影响因子：
11.2
作者：
Zylstra,S;Chen,FA;Ghosh,SK;Repasky,EA;Rao,U;Takita,H;Bankert,RB
通讯作者：
Bankert,RB
DOI：
--
发表时间：
1989-09
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
S. Kennel;L. Foote;R. Falcioni;A. Sonnenberg;C. Stringer;C. Crouse;M. Hemler
通讯作者：
S. Kennel;L. Foote;R. Falcioni;A. Sonnenberg;C. Stringer;C. Crouse;M. Hemler
新型人类整合素β亚基的克隆、一级结构和特性。
DOI：
10.1002/j.1460-2075.1990.tb08275.x
发表时间：
1990
期刊：
The EMBO journal
影响因子：
--
作者：
Ramaswamy,H;Hemler,ME
通讯作者：
Hemler,ME
DOI：
10.1073/pnas.83.22.8614
发表时间：
1986-11-01
影响因子：
11.1
作者：
SUZUKI, S;ARGRAVES, WS;RUOSLAHTI, E
通讯作者：
RUOSLAHTI, E