Age-related changes in dorsal root ganglia, circulating and vascular calcitonin gene-related peptide (CGRP) concentrations in female rats: effect of female sex steroid hormones.
Age-related changes in dorsal root ganglia, circulating and vascular calcitonin gene-related peptide (CGRP) concentrations in female rats: effect of female sex steroid hormones.
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DOI：
10.1016/j.neulet.2009.02.068
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发表时间：
2009-04-24
影响因子：
2.5
通讯作者：
Yallampalli C
中科院分区：
文献类型：
--
作者：
Gangula PR;Chauhan M;Reed L;Yallampalli C
本研究旨在探讨中年大鼠血浆和肠系膜动脉（抵抗动脉）中免疫反应性(I)降钙素基因相关肽（CGRP）含量是否降低，如果降低，性类固醇激素是否会增强中年雌性大鼠的I-CGRP。我们还研究了性类固醇激素治疗后中年雌性大鼠血管CGRP受体成分、降钙素受体样受体（CRLR）和受体活性修饰蛋白1 （RAMP1）是否升高。切除卵巢的成年（3月龄）和中年（10-12月龄）大鼠分别皮下注射雌二醇-17β (E2; 2 mg)、孕酮（P4; 5 mg）、E2 +P4 （2 mg + 20 mg）或安慰剂（对照组）。采用放射免疫法和Western blot法测定背根神经节（DRG）、阻力动脉和血浆中I-CGRP含量和受体组分。采用免疫荧光染色法测定耐药动脉中CRLR、RAMP1的细胞定位。我们的数据显示，与年轻对照组相比，中年雌性大鼠血浆和阻力动脉中I-CGRP含量显著（p < 0.05）降低。RAMP1和CRLR均集中在血管内皮和下层平滑肌细胞中。RAMP1在中年大鼠脉管系统中下降，而CRLR没有下降。去卵巢大鼠慢性灌注性类固醇激素：(1)DRG和血浆中I-CGRP显著（p < 0.05）升高；(2)RAMP1显著升高（p < 0.05），但抵抗动脉中CRLR未发生改变。这些数据表明雌性类固醇治疗可增强I-CGRP及其受体，从而调节老年雌性大鼠的血压。
The aim of the present study is to investigate whether immunoreactive (I) calcitonin gene-related peptide (CGRP) content is decreased in plasma and mesenteric arteries (resistance arteries) in middle-aged rats and if so, whether sex steroid hormones enhance I-CGRP in middle-aged female rats. We also examined whether vascular CGRP receptor components, calcitonin receptor like receptor (CRLR) and receptor activity modifying protein 1 (RAMP1) are elevated by sex steroid hormones treatment in middle-aged female rats. Young adult (3 months old) and middle-aged (10–12 months old) ovariectomized rats were treated subcutaneously with estradiol-17β (E2; 2 mg), progesterone (P4; 5 mg), E2 +P4 (2 mg + 20 mg) or placebo (control). Radioimmunoassay and Western blot analysis were performed to measure I-CGRP content and CGRP receptor components in dorsal root ganglia (DRG), in resistance arteries and in plasma. Immunofluorescent staining methods were employed to determine cellular localization of CRLR, RAMP1 in resistance arteries. Our data demonstrated that I-CGRP content was significantly (p < 0.05) lower in the plasma and resistance arteries of middle-aged female rats compared to young controls. Both RAMP1 and CRLR were concentrated in vascular endothelium and the underlying smooth muscle cells. RAMP1 but not CRLR appeared to be decreased in middle-aged rat vasculature. Chronic perfusion of sex steroid hormones to ovariectomized rats: (1) significantly (p < 0.05) elevated I-CGRP in the DRG and in the plasma, and (2) significantly elevated RAMP1 (p < 0.05) but did not alter CRLR in resistance arteries. These data suggest that female sex steroid treatment enhances I-CGRP and its receptors, and thus regulate the blood pressure in aged female rats.
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影响因子：
3.6
作者：
Gangula, PRR;Wimalawansa, SJ;Yallampalli, C
通讯作者：
Yallampalli, C
DOI：
10.1152/ajpheart.00140.2003
发表时间：
2004-01-01
影响因子：
4.8
作者：
Dong, YL;Vegiraju, S;Yallampalli, C
通讯作者：
Yallampalli, C
影响因子：
3.3
作者：
Ichikawa, Akiko;Sumino, Hiroyuki;Nitta, Kosaku
通讯作者：
Nitta, Kosaku
影响因子：
6.7
作者：
Manson, JM;Sammel, MD;Grisso, JA
通讯作者：
Grisso, JA
影响因子：
4.5
作者：
Blough, E. R.;Rice, K. M.;Wright, G. L.
通讯作者：
Wright, G. L.