Single-cell expression profiles of ACE2 and TMPRSS2 reveals potential vertical transmission and fetus infection of SARS-CoV-2.
Single-cell expression profiles of ACE2 and TMPRSS2 reveals potential vertical transmission and fetus infection of SARS-CoV-2.
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ACE2和TMPRSS2的单细胞表达曲线揭示了SARS-COV-2的潜在垂直传播和胎儿感染。
DOI：
10.18632/aging.104015
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发表时间：
2020-10-26
期刊：
影响因子：
--
通讯作者：
Leng Q
中科院分区：
文献类型：
--
作者：
Lü M;Qiu L;Jia G;Guo R;Leng Q
2019冠状病毒病（COVID-19）的发病率和死亡率取决于年龄。目前尚不清楚垂直严重急性呼吸综合征冠状病毒2（SARS-CoV-2）是否发生在怀孕期间，以及这种感染如何影响胎儿发育。在这里，我们使用公开的数据集对胎儿的胎盘和其他组织进行了单细胞转录组学分析，并与成人的组织进行了比较。我们的分析显示，一个非常小的比例的滋养层细胞表达血管紧张素I转化酶2（ACE 2）基因，这表明在怀孕期间SARS-CoV-2从母亲到胎儿垂直传播的可能性很低。我们发现胎儿肾上腺、心脏、肾脏和胃对SARS-CoV-2感染敏感，因为这些器官含有表达高水平ACE 2基因的细胞簇。特别是，与其他器官相比，肾上腺和肾脏中表达ACE 2的细胞簇的比例更高，也表达了跨膜丝氨酸蛋白酶2（TMPRSS 2）基因。令人惊讶的是，ACE 2表达II型肺泡（AT 2）等效细胞在胎儿肺中不存在。这与成年人的肺形成鲜明对比。由于ACE 2表达受各种条件（包括氧浓度、炎症和吸烟）调节，因此需要谨慎避免触发胎儿和胎盘组织中潜在的ACE 2表达。
Morbidity and mortality of coronavirus disease 2019 (COVID-19) is age-dependent. It remains unclear whether vertical severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) occurs during pregnancy and how such infection will affect fetal development. Here, we performed single-cell transcriptomic analysis of placenta and other tissues from fetuses in comparison with those from adults using public-available datasets. Our analysis revealed that a very small proportion of trophoblast cells expressed the Angiotensin I Converting Enzyme 2 (ACE2) gene, suggesting a low possibility of vertical transmission of SARS-CoV-2 from mother to fetus during pregnancy. We found that the fetal adrenal gland, heart, kidney and stomach were susceptible to SARS-CoV-2 infection, because these organs contained cell clusters that expressed high levels of the ACE2 gene. In particular, a higher proportion of ACE2-expressing cell clusters in the adrenal gland and kidney also expressed the Transmembrane Serine Protease 2 (TMPRSS2) gene compared with other organs. Surprisingly, ACE2-expressing type II alveolar (AT2) equivalent cells were absent in fetal lungs. This is in sharp contrast to adult lungs. As ACE2 expression is regulated by various conditions, including oxygen concentration, inflammation and smoking, caution is warranted to avoid triggering potential ACE2 expression in fetal and placental tissue.
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DOI：
10.3760/cma.j.cn112151-20200312-00193
发表时间：
2020-05-08
期刊：
Zhonghua bing li xue za zhi = Chinese journal of pathology
影响因子：
--
作者：
Yao, X H;Li, T Y;Bian, X W
通讯作者：
Bian, X W
影响因子：
64.8
作者：
Ge XY;Li JL;Yang XL;Chmura AA;Zhu G;Epstein JH;Mazet JK;Hu B;Zhang W;Peng C;Zhang YJ;Luo CM;Tan B;Wang N;Zhu Y;Crameri G;Zhang SY;Wang LF;Daszak P;Shi ZL
通讯作者：
Shi ZL
影响因子：
3.4
作者：
Hanley, Brian;Lucas, Sebastian B.;Osborn, Michael
通讯作者：
Osborn, Michael
影响因子：
30.3
作者：
Bayer A;Lennemann NJ;Ouyang Y;Bramley JC;Morosky S;Marques ET Jr;Cherry S;Sadovsky Y;Coyne CB
通讯作者：
Coyne CB
影响因子：
158.5
作者：
Guan, W.;Ni, Z.;Zhong, N.
通讯作者：
Zhong, N.