Adenovirus type 5 DNA-protein complexes from formaldehyde cross-linked cells early after infection.
Adenovirus type 5 DNA-protein complexes from formaldehyde cross-linked cells early after infection.
复制标题
感染后早期甲醛交联细胞中的 5 型腺病毒 DNA-蛋白质复合物。
DOI：
10.1016/s0042-6822(03)00194-6
复制
发表时间：
2003
期刊：
影响因子：
3.7
通讯作者：
Engel,DanielA
中科院分区：
文献类型：
--
作者：
Spector,DavidJ;Johnson,JeffreyS;Baird,NicholasL;Engel,DanielA
We report here the properties of viral DNA–protein complexes that purify with cellular chromatin following formaldehyde cross-linking of intact cells early after infection. The cross-linked viral DNA fractionated into shear-sensitive (S) and shear- resistant (R) components that were separable by sedimentation, which allowed independent characterization. The R component had the density and sedimentation properties expected for DNA–protein complexes and contained intact viral DNA. It accounted for about 50% of the viral DNA recovered at 1.5 h after infection but less than 20% by 4.5 h. The proportion of R component was independent of multiplicity of infection, even at less than one particle per cell. Viral hexon and protein VII, but not protein VI, were detected in the fractions containing the R component. These properties are consistent with those of partially uncoated virions associated with the nuclear envelope. A substantial proportion of the S component viral DNA had the same density as cellular chromatin. Protein VII was the most abundant viral protein present in gradient fractions that contained the S component. Complexes containing USF transcription factor cross-linked to the adenovirus major late promoter were detected by viral chromatin immunoprecipitation of the fractions containing S component. The S component probably contained uncoated nuclear viral DNA that assembles into early viral transcription complexes.
登录
查看更多内容
腺病毒 2 主要晚期启动子的转录物产生 3 共端 mRNA 的单个早期家族和五个晚期家族
影响因子：
64.5
作者：
A. Shaw;E. Ziff
通讯作者：
E. Ziff
紫外光诱导交联揭示腺病毒染色质的结构。
DOI：
10.1016/0006-291x(81)91591-6
发表时间：
1981
影响因子：
3.1
作者：
K. Sato;K. Hosokawa
通讯作者：
K. Hosokawa
腺病毒核蛋白的 DNase I 裂解
影响因子：
14.9
作者：
M. Fedor;E. Daniell
通讯作者：
E. Daniell
[3] 基因置换重组腺病毒的构建与分离
影响因子：
3.7
作者：
D. Spector;L. A. Samaniego
通讯作者：
L. A. Samaniego
5 型腺病毒 E1b 早期 RNA 合成激活中的顺式显性缺陷
影响因子：
5.4
作者：
C. Parks;D. Spector
通讯作者：
D. Spector