Effects of subretinal implant materials on the viability, apoptosis and barrier function of cultured RPE cells
Effects of subretinal implant materials on the viability, apoptosis and barrier function of cultured RPE cells
复制标题
视网膜下植入材料对培养RPE细胞活力、凋亡和屏障功能的影响
DOI：
10.1007/s00417-006-0296-4
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发表时间：
2006-04
影响因子：
2.7
通讯作者：
Pei, Wei-Hua
中科院分区：
文献类型：
--
作者：
Wu, Hui-Juan;Li, Xiao-Xin;Chen, Hong-Da;Dong, Jian-Qiang;Pei, Wei-Hua
背景视网膜下微光电二极管阵列（MPDA）是一种用于植入进行性光感受器细胞丧失患者视网膜下腔的视觉假体。本研究旨在探讨MPDA材料对体外培养的猪视网膜色素上皮细胞（retinal pigment epithelium，RPE）活力、凋亡和屏障功能的影响。将第三代培养物接种在用于MPDA和MPDA的不同材料上。四氮唑染料还原法（MTT）测定RPE细胞活力。流式细胞仪检测不同材料对RPE细胞凋亡的影响。将RPE细胞置于微孔滤膜上培养，通过测量实验分子的跨上皮阻力和通透性来确定屏障功能。结果所有方法的数据表明材料组与对照组之间无显著差异，结论本实验所用的MPDA材料对RPE细胞无直接毒性，不释放影响体外RPE屏障功能的有害可溶性因子。
BackgroundSubretinal microphotodiode array (MPDA) is a type of visual prosthesis used for the implantation in the subretinal space of patients with progressive photoreceptor cell loss. The present study aimed to evaluate the effect of materials for MPDA on the viability, apoptosis and barrier function of cultured pig retinal pigment epithelium (RPE) cells.MethodsPrimary culture of pig RPE cells was performed and 24 pig eyes were used to start RPE culture. The third passage of the cultures was plated on different materials for MPDA and MPDAs. The tetrazolium dye-reduction assay (MTT) was used to determine RPE cell viability. Flow cytometry was measured to indicate the apoptosis rates of RPE cells on different materials. RPE cells were also cultured on microporous filters, and the transepithelial resistance and permeability of the experimental molecule were measured to determine the barrier function.ResultsThe data from all the methods indicated no significant difference between the materials groups and the control group, and the materials tested showed good biocompatibility.ConclusionsThe materials for MPDA used in the present study had no direct toxicity to the RPE cells and did not release harmful soluble factors that affected the barrier function of RPE in vitro.
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一个自由访问的，基于证据的，客观的后囊分析系统；证据证明其有效性和可靠性。
影响因子：
2
作者：
通讯作者：
--
影响因子：
2.6
作者：
Zrenner, E
通讯作者：
Zrenner, E
影响因子：
3.2
作者：
Mannerström, M;Zorn-Kruppa, M;Tähti, H
通讯作者：
Tähti, H
DOI：
10.1002/(sici)1097-0320(19960601)24:2
发表时间：
1996-06
期刊：
Cytometry
影响因子：
--
作者：
C. Ferlini;S. Cesare;G. Rainaldi;W. Malorni;P. Samoggia;R. Biselli;A. Fattorossi
通讯作者：
C. Ferlini;S. Cesare;G. Rainaldi;W. Malorni;P. Samoggia;R. Biselli;A. Fattorossi
影响因子：
6.1
作者：
Y. Oshiro;J. Kachur;P. Balwierz;S. Won-Kim
通讯作者：
Y. Oshiro;J. Kachur;P. Balwierz;S. Won-Kim