Implementing Arbitrary CRNs Using Strand Displacing Polymerase
Implementing Arbitrary CRNs Using Strand Displacing Polymerase
复制标题
使用链置换聚合酶实现任意 CRN
DOI：
10.1007/978-3-030-26807-7_2
复制
发表时间：
2019
期刊：
影响因子：
13.3
通讯作者：
J. Reif
中科院分区：
文献类型：
--
作者：
Shalin Shah;Tianqi Song;Xin Song;Ming Yang;J. Reif
细胞和分子过程的调控通常涉及复杂的生化网络。合成核酸反应网络（包括基于酶和无酶的）可以系统地设计来近似复杂的生化过程。然而，大多数先前的实验协议的反应网络依赖于链置换杂交或限制性和外切酶酶促反应。这些合成系统通常要么反应速率慢，要么反应漏出。在这项工作中，我们提出了一种替代架构来实现任意反应网络，该网络完全基于具有非重叠I/O序列的链置换聚合酶反应。我们首先设计了一个简单的协议，近似任意单分子和双分子反应使用聚合酶链置换反应。然后，我们使用这些基本反应系统作为模块来展示我们的架构的三个大规模应用，包括自催化放大器，分子尺度共识协议和动态振荡系统。
The regulation of cellular and molecular processes typically involves complex biochemical networks. Synthetic nucleic acid reaction networks (both enzyme-based and enzyme-free) can be systematically designed to approximate sophisticated biochemical processes. However, most of the prior experimental protocols for reaction networks relied on either strand-displacement hybridization or restriction and exonuclease enzymatic reactions. These resulting synthetic systems usually suffer from either slow rates or leaky reactions. In this work, we propose an alternative architecture to implement arbitrary reaction networks, that is based entirely on strand-displacing polymerase reactions with non-overlapping I/O sequences. We first design a simple protocol that approximates arbitrary unimolecular and bimolecular reactions using polymerase strand displacement reactions. Then we use these fundamental reaction systems as modules to show three large-scale applications of our architecture, including an autocatalytic amplifier, a molecular-scale consensus protocol, and a dynamic oscillatory system.
影响因子：
16.6
作者：
Newman, Sharon;Stephenson, Ashley P.;Ceze, Luis
通讯作者：
Ceze, Luis
影响因子：
38.3
作者：
通讯作者：
--
影响因子：
16.6
作者：
Jiang, Yu Sherry;Bhadra, Sanchita;Li, Bingling;Ellington, Andrew D
通讯作者：
Ellington, Andrew D