Conversion of zonulae occludentes from tight to leaky strand type by introducing claudin-2 into Madin-Darby canine kidney I cells.
Conversion of zonulae occludentes from tight to leaky strand type by introducing claudin-2 into Madin-Darby canine kidney I cells.
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DOI：
10.1083/jcb.153.2.263
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发表时间：
2001-04-16
期刊：
影响因子：
--
通讯作者：
Tsukita S
中科院分区：
文献类型：
--
作者：
Furuse M;Furuse K;Sasaki H;Tsukita S
MDCK 细胞有两种细胞株：MDCK I 和 MDCK II。 MDCK I 细胞表现出比 MDCK II 细胞高得多的跨上皮电阻 (TER)，尽管它们具有相似数量的紧密连接 (TJ) 链。我们检查了这些细胞中TJ链的主要成分claudin的表达模式：claudin-1和-4在MDCK I和II细胞中均表达，而claudin-2的表达仅限于MDCK II细胞。然后将狗claudin-2 cDNA引入MDCK I细胞以模拟MDCK II细胞的claudin表达模式。有趣的是，稳定表达claudin-2（dCL2-MDCK I）的MDCK I克隆的TER值下降到MDCK II细胞的水平（下降>20倍）。相比之下，当狗claudin-3被引入MDCK I细胞时，其TER没有检测到变化。在小鼠上皮细胞 Eph4 中也获得了类似的结果。形态计量分析发现 dCL2-MDCK I 和对照 MDCK I 细胞之间的 TJ 密度或 TJ 链数量没有显着差异。这些发现表明，claudin-2 的添加显着降低了基于claudin-1/4 的单个TJ 链的紧密度，从而推测claudin 种类的组合和混合比例决定了单个TJ 链的阻隔性能。
There are two strains of MDCK cells, MDCK I and II. MDCK I cells show much higher transepithelial electric resistance (TER) than MDCK II cells, although they bear similar numbers of tight junction (TJ) strands. We examined the expression pattern of claudins, the major components of TJ strands, in these cells: claudin-1 and -4 were expressed both in MDCK I and II cells, whereas the expression of claudin-2 was restricted to MDCK II cells. The dog claudin-2 cDNA was then introduced into MDCK I cells to mimic the claudin expression pattern of MDCK II cells. Interestingly, the TER values of MDCK I clones stably expressing claudin-2 (dCL2-MDCK I) fell to the levels of MDCK II cells (>20-fold decrease). In contrast, when dog claudin-3 was introduced into MDCK I cells, no change was detected in their TER. Similar results were obtained in mouse epithelial cells, Eph4. Morphometric analyses identified no significant differences in the density of TJs or in the number of TJ strands between dCL2-MDCK I and control MDCK I cells. These findings indicated that the addition of claudin-2 markedly decreased the tightness of individual claudin-1/4–based TJ strands, leading to the speculation that the combination and mixing ratios of claudin species determine the barrier properties of individual TJ strands.
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DOI：
10.1083/jcb.147.1.185
发表时间：
1999-10-04
期刊：
The Journal of cell biology
影响因子：
--
作者：
Morita K;Sasaki H;Furuse M;Tsukita S
通讯作者：
Tsukita S
影响因子：
2.4
作者：
CLAUDE, P
通讯作者：
CLAUDE, P
通过表达突变型紧密连接膜蛋白，细胞旁通透性和跨上皮电阻的功能性分离以及顶端-基底外侧膜内扩散屏障的破坏。
影响因子：
7.8
作者：
Balda, M S;Whitney, J A;Flores, C;Gonzalez, S;Cereijido, M;Matter, K
通讯作者：
Matter, K
紧密连接链内和之间异质claudin物种相互作用的方式。
影响因子：
7.8
作者：
Furuse, M;Sasaki, H;Tsukita, S
通讯作者：
Tsukita, S
DOI：
10.1083/jcb.115.5.1449
发表时间：
1991-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Itoh M;Yonemura S;Nagafuchi A;Tsukita S;Tsukita S
通讯作者：
Tsukita S