Characterization of seed fatty acid accumulation in DELLA mutant lines of Arabidopsis
Characterization of seed fatty acid accumulation in DELLA mutant lines of Arabidopsis
复制标题
拟南芥 DELLA 突变系种子脂肪酸积累的表征
DOI：
10.1007/s10725-012-9775-2
复制
发表时间：
2013-05
影响因子：
4.2
通讯作者：
Jiang LX
中科院分区：
文献类型：
--
作者：
Ren Y;Zhou L;Chen XY;Jiang LX
拟南芥种子从胚胎到成熟期间积累了大量的脂肪酸（FA/s）。然而，决定 FA 积累的环境刺激和调节因素仍不清楚。在本研究中，我们研究了 DELLA（赤霉素转导途径中的负调控因子）在拟南芥种子油形成中的作用。比较DELLA ga1-3 rga-t2 rgl2-1(三重)、ga1-3 rga-t2 rgl1-1rgl2-1(四重)、ga1-3 gai-t6 rga-t2 rgl1-1 rgl 2-1(五重)和野生型(WT)的种子形态、千粒重、含油量和FA组成， Landsbergerecta（Ler）。观察到WT和五突变体之间上述性状的显着差异。与 WT 相比，五核种子的特点是种子油（以 μg/mg 计）减少了 22%，以 FA/种子计增加了 10%（以 FA/种子计），并且 FA 组成发生了变化。一般来说，五果和野生型发育种子（花后 2-16 天，DAA）中 FA 积累的动态没有明显不同。然而，与 WT 相比，6 DAA 的五粒种子中 FA 积累量更高。 RNA凝胶印迹表明脂肪酸去饱和酶基因在种子和叶子中表达。五突变体和WT之间基因的不同转录水平揭示了造成所观察到的差异的部分原因。据我们所知，这是第一份关于 DELLA 对开花植物种子油形成动态过程影响的报告。
Arabidopsis seeds accumulate considerable fatty acids (FA/s) from embryo until maturity. The environmental stimuli and the regulatory factors that determine FA accumulation, however, remain unclear. In this study, we investigated the role of DELLAs, the negative regulatory factors in the gibberellin transduction pathway, in seed oil formation in Arabidopsis. Seed morphology, weight-per-thousand seeds, oil content, and FA composition were compared among the seeds of theDELLAga1-3 rga-t2 rgl2-1(triple),ga1-3 rga-t2 rgl1-1rgl2-1(quadruple),ga1-3 gai-t6 rga-t2 rgl1-1 rgl 2-1(penta), and wild type (WT), Landsbergerecta(Ler). Significant differences between the WT and thepentamutant for the above traits were observed. Compared with WT, thepentaseeds were characterized by a decrease of 22 % seed oil in term of μg/mg, an increment of 10 % seed oil in term of FA/seed, and an alteration of FA composition. In general, the dynamics of FA accumulation in thepentaand WT developing seeds (2–16 days after anthesis, DAA) were not obviously different. However, there was higher FA accumulation inpentaseeds from 6 DAA compared with WT. RNA gel blotting indicated that the fatty acid desaturase genes were expressed in seeds as well as in leaves. The different transcript levels of the genes between thepentamutant and WT unveiled a part of the reasons accounting for the differences observed. To our knowledge, this is the first report on the effect of DELLAs on the dynamic process of seed oil formation of a flowering plant.
登录
查看更多内容
影响因子：
2.1
作者：
V. Zheljazkov;B. Vick;B. Baldwin;N. Buehring;T. Astatkie;B. Johnson
通讯作者：
V. Zheljazkov;B. Vick;B. Baldwin;N. Buehring;T. Astatkie;B. Johnson
影响因子：
7.4
作者：
Hobbs, DH;Flintham, JE;Hills, MJ
通讯作者：
Hills, MJ
影响因子：
7.4
作者：
Heilmann, I;Mekhedov, S;Shanklin, J
通讯作者：
Shanklin, J
DOI：
--
发表时间：
--
期刊：
--
影响因子：
--
作者：
K. Livak;Thomas D. Schmittgen
通讯作者：
K. Livak;Thomas D. Schmittgen
DOI：
10.1073/pnas.94.10.5473
发表时间：
1997-05-13
影响因子：
11.1
作者：
McConn, M;Creelman, RA;Browse, J
通讯作者：
Browse, J