Development of decellularized human umbilical arteries as small-diameter vascular grafts.
Development of decellularized human umbilical arteries as small-diameter vascular grafts.
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DOI：
10.1089/ten.tea.2008.0526
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发表时间：
2009-09
影响因子：
--
通讯作者：
Niklason LE
中科院分区：
文献类型：
--
作者：
Gui L;Muto A;Chan SA;Breuer CK;Niklason LE
在过去的几十年里，开发一种用于重建手术的组织工程化小直径(<；6 mm)血管移植物一直是一个挑战。本研究旨在开发一种脱细胞的脐动脉，并评价其成分、内皮细胞的相容性、力学性能和体内稳定性，以期作为一种小直径的血管移植物。人脐动脉在CHAPS和十二烷基硫酸钠缓冲液中孵育，然后在内皮生长介质-2中孵育，分离并脱细胞。去细胞的脐动脉完全没有细胞和核物质，同时保留了细胞外胶原基质的完整性。脱细胞脐动脉的机械强度通过其体外破裂压力来评估，与其天然形式相比没有明显的变化。脱细胞的脐动脉支持内皮黏附，如单层人脐静脉内皮细胞的再内皮化所示。此外，作为腹主动脉间置移植物植入裸鼠体内的脱细胞血管在机械上保持完好，通畅时间长达8周。脱细胞的人脐动脉保存细胞外基质，支持内皮化，并在体内保留功能长达8周。这些特性提示去细胞脐动脉作为小直径血管移植物的潜在用途。
Developing a tissue-engineered small-diameter (<6 mm) vascular graft for reconstructive surgery has remained a challenge for the past several decades. This study was conducted to develop a decellularized umbilical artery and to evaluate its composition, endothelial cell compatibility, mechanical properties, and in vivo stability for potential use as a small-diameter vascular graft. Human umbilical arteries were isolated and decellularized by incubation in CHAPS and sodium dodecyl sulfate buffers followed by incubation in endothelial growth media-2. Decellularized umbilical arteries were completely devoid of cellular and nuclear material while retaining the integrity of extracellular collagenous matrix. The mechanical strength of the decellularized umbilical artery as assessed by its burst pressure in vitro showed no significant change from its native form. Decellularized umbilical arteries supported endothelial adherence as indicated by the re-endotheliazation with a monolayer of human umbilical vein endothelial cells. Furthermore, decellularized vessels that were implanted into nude rats as abdominal aorta interposition grafts remained mechanically intact and patent for up to 8 weeks. Decellularized human umbilical arteries preserved the extracellular matrix, supported endothelialization, and retained function in vivo for up to 8 weeks. These properties suggest the potential use of decellularized umbilical arteries as small-diameter vascular grafts.
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