Exoproteome analysis of Clostridium cellulovorans in natural soft-biomass degradation.
Exoproteome analysis of Clostridium cellulovorans in natural soft-biomass degradation.
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DOI：
10.1186/s13568-014-0089-9
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发表时间：
2015-12
期刊：
影响因子：
3.7
通讯作者：
Ueda M
中科院分区：
文献类型：
--
作者：
Esaka K;Aburaya S;Morisaka H;Kuroda K;Ueda M
纤维素梭菌是一种厌氧纤维素分解细菌，能够有效降解各种类型的软质生物质。其出色的降解能力源自优化蛋白质复合物（纤维素体）的组成以及根据底物类型生产非纤维素体蛋白质。在这项研究中，我们进行了定量蛋白质组分析，以确定由 C. cellulovorans 产生的用于降解几种类型的天然软生物质的细胞外蛋白质的变化。 C. cellulovorans 在含有甘蔗渣、玉米胚芽、稻草（天然软生物质）或纤维二糖（对照）的培养基中培养。使用同量异位标签方法和配备长整体硅胶毛细管柱/质谱仪的液相色谱仪，我们鉴定了培养物上清液中的 372 个蛋白质。其中，我们重点研究了纤维素体和非纤维素体来源的 77 种糖化相关蛋白。统计分析表明，其中 18 种蛋白质是在天然软质生物质降解过程中专门产生的。有趣的是，蛋白质 Clocel_3197 被发现并通常参与所研究的每种天然软生物质的降解。除了其已知的代谢功能之外，该蛋白质还可以执行有助于有效降解天然软生物质的功能。本文的在线版本 (doi:10.1186/s13568-014-0089-9) 包含补充材料，可供授权用户使用。
Clostridium cellulovorans is an anaerobic, cellulolytic bacterium, capable of effectively degrading various types of soft biomass. Its excellent capacity for degradation results from optimization of the composition of the protein complex (cellulosome) and production of non-cellulosomal proteins according to the type of substrates. In this study, we performed a quantitative proteome analysis to determine changes in the extracellular proteins produced by C. cellulovorans for degradation of several types of natural soft biomass. C. cellulovorans was cultured in media containing bagasse, corn germ, rice straw (natural soft biomass), or cellobiose (control). Using an isobaric tag method and a liquid chromatograph equipped with a long monolithic silica capillary column/mass spectrometer, we identified 372 proteins in the culture supernatant. Of these, we focused on 77 saccharification-related proteins of both cellulosomal and non-cellulosomal origins. Statistical analysis showed that 18 of the proteins were specifically produced during degradation of types of natural soft biomass. Interestingly, the protein Clocel_3197 was found and commonly involved in the degradation of every natural soft biomass studied. This protein may perform functions, in addition to its known metabolic functions, that contribute to effective degradation of natural soft biomass. The online version of this article (doi:10.1186/s13568-014-0089-9) contains supplementary material, which is available to authorized users.
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