Nanostructured calcium phosphate coatings on magnesium alloys: characterization and cytocompatibility with mesenchymal stem cells.
Nanostructured calcium phosphate coatings on magnesium alloys: characterization and cytocompatibility with mesenchymal stem cells.
复制标题
DOI：
10.1007/s10856-015-5512-5
复制
发表时间：
2015-05
期刊：
影响因子：
--
通讯作者：
Liu H
中科院分区：
文献类型：
--
作者：
Iskandar ME;Aslani A;Tian Q;Liu H
本文报道了纳米结构磷酸钙 (nCaP) 在镁钇合金基底上的沉积和表征及其与骨髓间充质干细胞 (BMSC) 的细胞相容性。使用专有的跨音速粒子加速工艺将 nCaP 涂层沉积在镁和镁钇合金基材上，以达到调节基材降解和 BMSC 粘附的双重目的。使用扫描电子显微镜和定量图像分析工具分析表面形态和特征尺寸。分别使用能量色散X射线光谱和X射线衍射分析表面元素组成和相。沉积的 nCaP 涂层显示出均匀的颗粒表面，主要特征尺寸为长轴 200-500 nm，短轴 100-300 nm，Ca/P 原子比为 1.5-1.6。羟基磷灰石是 nCaP 涂层中确定的主要相。 nCaP 涂层对样品降解和 BMSC 行为的调节作用取决于基底成分和表面条件。 BMSCs 体外直接培养表明，多种因素，包括表面组成和形貌，以及降解引起的培养基组成变化，影响细胞在样品表面的直接粘附，以及同一培养物中样品周围的间接粘附。碱性pH值作为镁降解的指标，在BMSC粘附和形态中发挥作用，但不是唯一因素。需要进行更多研究来阐明 BMSC 对每个影响因素的反应。
This article reports the deposition and characterization of nanostructured calcium phosphate (nCaP) on magnesium–yttrium alloy substrates and their cytocompatibility with bone marrow derived mesenchymal stem cells (BMSCs). The nCaP coatings were deposited on magnesium and magnesium–yttrium alloy substrates using proprietary transonic particle acceleration process for the dual purposes of modulating substrate degradation and BMSC adhesion. Surface morphology and feature size were analyzed using scanning electron microscopy and quantitative image analysis tools. Surface elemental compositions and phases were analyzed using energy dispersive X-ray spectroscopy and X-ray diffraction, respectively. The deposited nCaP coatings showed a homogeneous particulate surface with the dominant feature size of 200–500 nm in the long axis and 100–300 nm in the short axis, and a Ca/P atomic ratio of 1.5–1.6. Hydroxyapatite was the major phase identified in the nCaP coatings. The modulatory effects of nCaP coatings on the sample degradation and BMSC behaviors were dependent on the substrate composition and surface conditions. The direct culture of BMSCs in vitro indicated that multiple factors, including surface composition and topography, and the degradation-induced changes in media composition, influenced cell adhesion directly on the sample surface, and indirect adhesion surrounding the sample in the same culture. The alkaline pH, the indicator of Mg degradation, played a role in BMSC adhesion and morphology, but not the sole factor. Additional studies are necessary to elucidate BMSC responses to each contributing factor.
登录
查看更多内容
影响因子：
14
作者：
Kim, HW;Kim, HE;Salih, V
通讯作者：
Salih, V
影响因子：
4.9
作者：
Mueller, Wolf-Dieter;Fernandez Lorenzo de Mele, Monica;Zeddies, Miriam
通讯作者：
Zeddies, Miriam
影响因子：
3.6
作者：
Galli, Silvia;Naito, Yoshihito;Jimbo, Ryo
通讯作者：
Jimbo, Ryo
影响因子：
3.7
作者：
LO, CM;KEESE, CR;GIAEVER, I
通讯作者：
GIAEVER, I
影响因子：
4.5
作者：
Aghion, Eli;Gueta, Yael;Bronfin, Boris
通讯作者：
Bronfin, Boris