Interaction with diurnal and circadian regulation results in dynamic metabolic and transcriptional changes during cold acclimation in Arabidopsis.
Interaction with diurnal and circadian regulation results in dynamic metabolic and transcriptional changes during cold acclimation in Arabidopsis.
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DOI：
10.1371/journal.pone.0014101
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发表时间：
2010-11-23
期刊：
影响因子：
3.7
通讯作者：
Hannah MA
中科院分区：
文献类型：
--
作者：
Espinoza C;Degenkolbe T;Caldana C;Zuther E;Leisse A;Willmitzer L;Hincha DK;Hannah MA
在植物中，冷和昼夜节律调节基因之间有很大的重叠，在拟南芥中，我们已经表明冷(4℃)影响时钟振荡器基因的表达。然而，对寒冷反应的昼夜和/或昼夜节律调节的意义，特别是对代谢途径的意义及其生理相关性缺乏更广泛的洞察力。在这里，我们使用微阵列和气相色谱-质谱仪对转录本和初级代谢物进行了综合分析。正如预期的那样，在冷驯化过程中，昼夜调节基因的表达受到了很大的影响。我们的数据表明，时钟功能在转录水平的中断延伸到代谢调节。大约80%表现出昼夜循环的代谢物在冷处理期间保持这种状态。特别是，麦芽糖的含量在寒冷中显示出夜间的大量增加。然而，在自由运转的条件下，麦芽糖是唯一在寒冷中保持振荡的代谢物。此外，尽管淀粉在冷驯化过程中积累，但我们发现它在夜间仍然被降解，这表明在冷驯化的初始阶段，麦芽糖和淀粉分解的作用超出了以前所证明的意义。一些常规的冷诱导代谢物，如γ-氨基丁酸、半乳糖醇、棉籽糖和腐胺，其水平表现出昼夜和昼夜振荡，编码其生物合成酶的转录本也经常在冷诱导之前循环，这与转录调控一致。然而，其他冷反应代谢物的积累，如高丝氨酸、蛋氨酸和麦芽糖，没有一致的转录调节，这意味着代谢重组涉及复杂的转录和转录后机制。这些数据证明了在正确的昼夜背景下理解冷适应的重要性，并进一步支持了我们在昼夜突变中发现的受损的冷适应。
In plants, there is a large overlap between cold and circadian regulated genes and in Arabidopsis, we have shown that cold (4°C) affects the expression of clock oscillator genes. However, a broader insight into the significance of diurnal and/or circadian regulation of cold responses, particularly for metabolic pathways, and their physiological relevance is lacking. Here, we performed an integrated analysis of transcripts and primary metabolites using microarrays and gas chromatography-mass spectrometry. As expected, expression of diurnally regulated genes was massively affected during cold acclimation. Our data indicate that disruption of clock function at the transcriptional level extends to metabolic regulation. About 80% of metabolites that showed diurnal cycles maintained these during cold treatment. In particular, maltose content showed a massive night-specific increase in the cold. However, under free-running conditions, maltose was the only metabolite that maintained any oscillations in the cold. Furthermore, although starch accumulates during cold acclimation we show it is still degraded at night, indicating significance beyond the previously demonstrated role of maltose and starch breakdown in the initial phase of cold acclimation. Levels of some conventional cold induced metabolites, such as γ-aminobutyric acid, galactinol, raffinose and putrescine, exhibited diurnal and circadian oscillations and transcripts encoding their biosynthetic enzymes often also cycled and preceded their cold-induction, in agreement with transcriptional regulation. However, the accumulation of other cold-responsive metabolites, for instance homoserine, methionine and maltose, did not have consistent transcriptional regulation, implying that metabolic reconfiguration involves complex transcriptional and post-transcriptional mechanisms. These data demonstrate the importance of understanding cold acclimation in the correct day-night context, and are further supported by our demonstration of impaired cold acclimation in a circadian mutant.
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影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
DOI：
10.1073/pnas.0406069101
发表时间：
2004-10-19
影响因子：
11.1
作者：
Cook, D;Fowler, S;Thomashow, MF
通讯作者：
Thomashow, MF
影响因子：
7.4
作者：
Fowler, SG;Cook, D;Thomashow, ME
通讯作者：
Thomashow, ME
影响因子：
3.6
作者：
Benkeblia, Noureddine;Shinano, Takuro;Osaki, Mitsuru
通讯作者：
Osaki, Mitsuru
影响因子：
64.8
作者：
Doyle, MR;Davis, SJ;Amasino, RM
通讯作者：
Amasino, RM