Catalase and superoxide dismutase-2 enhance survival and protect ovaries during overwintering diapause in the mosquito Culex pipiens.
Catalase and superoxide dismutase-2 enhance survival and protect ovaries during overwintering diapause in the mosquito Culex pipiens.
复制标题
DOI：
10.1016/j.jinsphys.2011.01.012
复制
发表时间：
2011-05
影响因子：
2.2
通讯作者：
Denlinger, David L.
中科院分区：
文献类型：
--
作者：
Sim, Cheolho;Denlinger, David L.
延寿和抗逆性是滞育的两个重要特征，是成功越冬的关键。我们提出了几条证据，表明编码两种抗氧化酶--过氧化氢酶和超氧化物歧化酶-2的基因在蚊子越冬成虫滞育期间产生这些特征方面起关键作用。在年轻的滞育女性中，过氧化氢酶和超氧化物歧化酶-2的表达显著高于相同年龄的非滞育女性。抑制过氧化氢酶，而不是超氧化物歧化酶-2，导致卵巢损伤增加，这从卵巢卵泡细胞的凋亡迹象中得到了证明。当使用RNAi抑制过氧化氢酶或超氧化物歧化酶-2的水平时，成年动物的存活时间被缩短。这些结果表明，这两种抗氧化剂在促进滞育雌性动物存活方面特别重要，而过氧化氢酶的升高也有助于保护卵巢。此外，针对叉头转录因子(FOXO)的RNAi，被认为是这些抗氧化剂基因上游的一个基因，导致过氧化氢酶和超氧化物歧化酶-2的抑制。与FOXO的连锁表明，编码这两种抗氧化剂的基因是受该转录因子调控的重要基因网络的组成部分。
Lifespan extension and stress resistance are two important features of diapause that are essential for successful overwintering. We present several lines of evidence suggesting that genes encoding two antioxidant enzymes, catalase and superoxide dismutase-2, are critical in generating these characteristics during diapause in overwintering adults of the mosquito Culex pipiens. Expression of both catalase and sod-2 was dramatically higher in young diapausing females than in their nondiapausing counterparts at the same age. Suppression of catalase, but not sod-2, resulted in increased damage to the ovaries, as evidenced by signs of apoptosis in ovarian follicle cells. Adult survival time was shortened when levels of either catalase or sod-2 were suppressed using RNAi. Together these results imply that these two antioxidants are particularly important in promoting survival in diapausing females, while elevation of catalase also contributes to protection of the ovaries. In addition, RNAi directed against forkhead transcription factor (foxo), a gene thought to be upstream of the genes encoding these antioxidants, resulted in suppression of both catalase and sod-2. The linkage with FOXO suggests that the genes encoding these two antioxidants are components of an important gene network regulated by this transcription factor.
登录
查看更多内容
影响因子：
7.8
作者：
McElwee, J;Bubb, K;Thomas, JH
通讯作者：
Thomas, JH
影响因子：
4.6
作者：
Gershman, Boris;Puig, Oscar;Garofalo, Robert S.
通讯作者：
Garofalo, Robert S.
影响因子：
2.2
作者：
KOBAYASHI, M;ISHIKAWA, H
通讯作者：
ISHIKAWA, H
影响因子：
2.1
作者：
MITCHELL, CJ;BRIEGEL, H
通讯作者：
BRIEGEL, H
影响因子：
1.3
作者：
Denlinger, David L.
通讯作者：
Denlinger, David L.