In vitro analysis of the interaction between yeast fimbrin and actin: A model for a mechanism of allele-specific suppression
In vitro analysis of the interaction between yeast fimbrin and actin: A model for a mechanism of allele-specific suppression
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酵母菌丝蛋白和肌动蛋白之间相互作用的体外分析:等位基因特异性抑制机制的模型
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发表时间:
1996
期刊:
影响因子:
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通讯作者:
T. Sandrock
中科院分区:
文献类型:
--
作者:
T. Sandrock
11 CHAPTER ONE: Introduction 13 PART I: Introduction to the Cytoskeleton 13 Actin 13 Actin Localization in Yeast 14 Identification of Proteins That Genetically Interact with Actin 15 Yeast Fimbrin, Sac6p 16 PART II: Allele-Specific Suppression 19 Molecular Mechanisms of Allele-Specific Suppression 19 Allele-Specific High Copy Suppression 24 Allele-Specific Reciprocal Suppression 25 Cycling-Suppressor Screens 26 Defining Domains 27 Dominant vs. Recessive Suppressors 28 Null Allele-Specific Suppressors 29 Null Allele-Specific Intergenic Noncomplementation 29 Intragenic Suppressors 30 Other Examples 31 Other Techniques 31 Concluding Remarks 32 CHAPTER TWO: Materials and Methods 35 Sac6p Purification 35 Protein Sequence Analysis 36 CNBr Cleavage for Sequencing 37 Trypsin Digestion 38 Bmding and Bundling Assays for Calcium Experiment 38 Anti-Sac6p /uitibody Purification 39 Gel Filtration 40
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